Somatostatin receptor agonist formulations

ABSTRACT

The present invention relates to compositions forming a low viscosity mixture of:
         a) 20-50 wt. % of at least one diacyl glycerol;   b) 20-54 wt. % of at least one phosphatidyl choline (PC);   c) 5-15 wt. % of at least one biocompatible, organic mono-alcoholic solvent;   d) 1 to 20 wt. % polar solvent   e) 5 to 150 mg/ml of at least one peptide somatostatin receptor agonist comprising pasireotide;   f) optionally at least one antioxidant;
 
wherein the ratio of components a:b is in the range 40:60 to 54:46;
 
wherein the pre-formulation forms, or is capable of forming, at least one liquid crystalline phase structure upon contact with excess aqueous fluid.
       

     The invention further relates to methods of treatment comprising administration of such compositions, and to pre-filled administration devices and kits containing the formulations.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a continuation of U.S. application Ser. No.14/401,559, filed on Nov. 17, 2014, which is a 371 application ofInternational Application No. PCT/EP2013/060739, filed on May 24, 2013,which is a 371 application of International Application No.PCT/EP2012/059917, filed on May 25, 2012, which claims priority to U.S.Application No. 61/730,613, filed on Nov. 28, 2012, which isincorporated herein by reference.

SEQUENCE LISTING

The Sequence Listing, submitted herewith through Patent Center as aneXtensible Markup Language file (2023 Apr. 25_Sequence listing.xml,created on Apr. 25, 2023, 2,729 bytes), is incorporated by reference.

FIELD OF THE INVENTION

The present invention relates to formulation precursors(pre-formulations) for the in situ generation of compositions for thecontrolled release of peptide active agents, and methods of treatmentwith such formulations. In particular, the invention relates tohigh-loading pre-formulations of amphiphilic components and at least onepeptide active agent comprising pasireotide for parenteral application,which undergo phase transition upon exposure to aqueous fluids, such asbody fluids, thereby forming a controlled release composition.

BACKGROUND TO THE INVENTION

Many bioactive agents including pharmaceuticals, nutrients, vitamins andso forth have a “functional window”. That is to say that there is arange of concentrations over which these agents can be observed toprovide some biological effect. Where the concentration in theappropriate part of the body (e.g. locally or as demonstrated by serumconcentration) falls below a certain level, no beneficial effect can beattributed to the agent. Similarly, there is generally an upperconcentration level above which no further benefit is derived byincreasing the concentration. In some cases increasing the concentrationabove a particular level results in undesirable or even dangerouseffects.

Some bioactive agents have a long biological half-life and/or a widefunctional window and thus may be administered occasionally, maintaininga functional biological concentration over a substantial period of time(e.g. 6 hours to several days). In other cases the rate of clearance ishigh and/or the functional window is narrow and thus to maintain abiological concentration within this window regular (or even continuous)doses of a small amount are required. This can be particularly difficultwhere non-oral routes of administration (e.g. parenteral administration)are desirable or necessary, since self-administration may be difficultand thus cause inconvenience and/or poor compliance. In such cases itwould be advantageous for a single administration to provide activeagent at a therapeutic level over the whole period during which activityis needed.

There is an enormous potential in the use of peptides (includingproteins) for treating various disease states, as well as in prophylaxisand in improving general health and well-being of subjects. However, theperformance of administered peptide agents is generally limited due topoor bioavailability, which in turn is caused by the rapid degradationof peptides and proteins in biological fluids. This increases the dosewhich must be administered and in many cases restricts the effectiveroutes of administration. These effects are further exaggerated by theoften limited permeability of peptides and proteins across biologicalmembranes.

Peptides and proteins that are administered to the mammalian body (e.g.orally, intramuscularly etc.) are subject to degradation by variousproteolytic enzymes and systems present throughout the body. Well knownsites of peptidase activity include the stomach (e.g. pepsin), and theintestinal tract (e.g. trypsin, chymotrypsin, and others) but otherpeptidases (e.g. aminopeptidases, carboxypeptidases, etc.) are foundthroughout the body. Upon oral administration, gastric and intestinaldegradation reduces the amount of peptide or protein which potentiallycould be absorbed through the intestinal surface lining and therebydecreases their bioavailability. Similarly, free peptides and proteinsin the mammalian blood stream are also subject to enzymatic degradation(e.g. by plasma proteases etc.).

Some patients undergoing treatment will typically require a therapeuticdose to be maintained for a considerable period and/or ongoing treatmentfor many months or years. Thus a depot system allowing loading andcontrolled release of a larger dose over a longer period would offer aconsiderable advantage over conventional delivery systems.

Peptides may be delivered by systems such as the Alkermes Medisorb®delivery system consisting of microspheres of biodegradable polymers.Such polymer microsphere formulations must generally be administered bymeans of a sizable needle, typically of 20-gauge or wider. This isnecessary as a result of the nature of the polymeric dosing systemsused, which are typically polymer suspensions.

Evidently, it would be an advantage to provide a system of lowviscosity, such as a homogeneous solution, dispersion of fine particles,or L₂ phase, which could be administered easily through a narrow needle,thus decreasing the discomfort of the patient during the procedure. Thisease of administration is particularly significant where patients willbe on a self-administration regime and may already be self-administeringseveral times each day. Providing a sustained formulation with aduration of a few days, but which is sufficiently complex to administerthat it requires treatment by a healthcare professional will not be anadvantage to all patients over twice-daily or daily self-administration,and is likely to be more costly. Providing a formulation which givessufficiently long duration to justify a visit to a health professionalfor administration and/or a preparation which can be self-administered,and reducing preparation time of health-care professionals or patientsprior to the actual administration are all important issues.

The poly-lactate, poly-glycolate and poly-lactate-co-glycolate polymerstypically used for degrading slow-release formulations are also thecause of some irritation in at least some patients. In particular, thesepolymers typically contain a certain proportion of acidic impuritiessuch as lactic and glycolic acid, which will irritate the injection siteon administration. When the polymer then breaks down, lactic acid andglycolic acid are the degradation products so that further irritation iscaused. As a result of the combined effects of wide-needleadministration and irritant contents, discomfort at the site ofadministration and the formation of connective scar tissue are greaterthan desirable.

From a drug delivery point of view, polymer depot compositions generallyhave the disadvantage of accepting only relatively low drug loads andhaving a “burst/lag” release profile. The nature of the polymericmatrix, especially when applied as a solution or pre-polymer, causes aninitial burst of drug release when the composition is firstadministered. This is followed by a period of low release, while thedegradation of the matrix begins, followed finally by an increase in therelease rate to the desired sustained profile. This burst/lag releaseprofile can cause the in vivo concentration of active agent to burstabove the functional window immediately following administration, andthen drop back through the bottom of the functional window during thelag period before reaching a sustained functional concentration for aperiod of time. Evidently, from a functional and toxicological point ofview this burst/lag release profile is undesirable and could bedangerous. It may also limit the equilibrium concentration which can beprovided due to the danger of adverse effects at the “peak” point. Thepresence of a lag phase may furthermore require supplementary dosingwith repeat injections during the start-up period of depot treatment inorder to maintain a therapeutic dose while the concentrations of activeprovided from the depot are sub-functional. For certain polypeptides inparticular, it would be advantageous to minimise the immediate “burst”effect upon administration of a composition in order to avoid sideeffects such as hypoglycaemia.

One class of peptide hormones which benefits particularly from a very“low burst”, stable in vivo concentration are Somatostatin analoguessuch as Pasireotide (SOM230). In vivo testing suggests that thesepeptides are particularly beneficial when maintained at a steady plasmaconcentration and as a regulatory hormone, Pasireotide is particularlylikely to benefit from a stable plasma level. This not only suggeststhat a depot composition would be an advantage to avoid “spikes” inconcentration upon administration and/or repeated daily dosing, butfurthermore that such a depot composition should have as flat a releaseprofile as possible during the therapeutic period.

Controlled-release formulations are typically generated frombio-compatible polymers in the form of, for example, implants orinjectable beads. The current leading formulation of Pasireotide, forexample (Pasireotide LAR) comprises microparticles of poly(D,L-lactide-co-glycolide). Polymer microsphere formulations mustgenerally be administered by means of a sizable needle, typically of20-gauge or wider. This is necessary as a result of the nature of thepolymeric dosing systems used, which are typically polymer suspensions.It would be an advantage to provide a system of low viscosity, such as ahomogeneous solution, dispersion of fine particles, or L₂ phase, whichcould be administered easily through a narrow needle, thus decreasingthe discomfort of the patient during the procedure. Ease ofadministration is particularly significant when patients will beself-administering but also reduces the burden on healthcareprofessionals when they are conducting the administration.

The manufacture of PLGA microbeads and suspensions is additionally aconsiderable difficulty with certain existing depot systems. Inparticular, since the beads are particulate they cannot generally besterile-filtered and furthermore, since the PLGA copolymer melts atelevated temperature, they cannot be heat-treated for sterility. As aresult, the complex manufacturing process must be conducted aseptically.

Further issues with biodegradable polymer microspheres include complexreconstitution prior to injection and limited storage stability, dueboth to aggregation and degradation of the delivery system and/oractive.

A lipid-based, slow-release composition has been described for certainpeptides. For example, WO2006/131730 discloses a lipid depot system forGLP-1 and analogues thereof. This is a highly effective formulation, butthe concentration of active agent which can be included in theformulation is limited by its solubility. Evidently, a higherconcentration of active agent allows for the possibility of longerduration depot products, products maintaining a higher systemicconcentration, and products having a smaller injection volume, all ofwhich factors are of considerable advantage under appropriatecircumstances. It would thus be of considerable value to establish a wayby which higher concentrations of active agents could be included in alipid-based depot formulation and to identify combinations of activeagent and delivery system which are particularly effective from thepoint of view of loading, stability, manufacturing and/or controlledrelease.

The present inventors have now established that by providing apre-formulation comprising at least one neutral diacyl glycerol, atleast one phosphatidyl choline, at least one biocompatible organicmono-alcoholic solvent, at least one polar solvent, at least one peptideactive agent comprising pasireotide (SOM230) and optionally at least oneantioxidant in a low viscosity phase, such as molecular solution or L₂(reversed micellar) phase, a pre-formulation may be generated addressingmany of the shortfalls of known depot formulations, and which may beapplied to provide a controlled release of the pasireotide active agent.By use of specific components in carefully selected ratios, and inparticular with a mixture of pasireotide, an alcohol and a polarsolvent, a depot formulation can be generated having a combination ofproperties exceeding the performance even of previous lipidcontrolled-release compositions and providing an advantage over knowncompositions such as pasireotide LAR.

In particular, the pre-formulation shows a highly advantageous releaseprofile, is easy to manufacture, may be sterile-filtered, has lowviscosity (allowing easy and less painful administration typicallythrough a narrow needle), allows a high level of bioactive agent to beincorporated (thus potentially allowing a smaller amount of compositionand/or active agent to be used), requires shallow injection and/or formsa desired non-lamellar depot composition in vivo having a “non-burst”release profile. The compositions are also formed from materials thatare non-toxic, biotolerable and biodegradable, which can be administeredby i.m., or s.c. injection and are suitable for self-administration. Thepre-formulation may additionally have a very low level of irritation oninjection and in preferred cases causes no irritation at the injectionsite (including transient irritation).

Certain of the formulations of the present invention generate anon-lamellar liquid crystalline phase following administration. The useof non-lamellar phase structures (such as non-lamellar liquidcrystalline phases) in the delivery of bioactive agents is nowrelatively well established. A most effective lipid depot system isdescribed in WO2005/117830, and a highly preferred lipid depot isdescribed in that document. However, there remains scope for achievingdepot formulations having improved performance in several respects andin particular, surprising improvements can be achieved by carefulselection and optimisation of the range of components and proportionsdisclosed in previous work.

Advantages of the compositions of the present invention over polymerformulations, such as PLGA microspheres, include the ease of manufacture(including sterilization), handling and use properties combined with lowinitial release (“non-burst profile”) of active agent. This may bedefined such that the area under a plasma concentration against time thecurve during the first 24 hours of a one-month dosing period is lessthan 20% of the area under the curve for the entire curve (measured orextrapolated from time 0 to infinity or from time 0 to the last samplingtime point), more preferably less than 15% and most preferable less than10%. Furthermore, it may be defined such that the maximum plasmaconcentration of active agent in vivo following injection of thepre-formulation (Cmax) is no more than 10 times, preferably no more than8 times and most preferably no more than 5 times the average plasmaconcentration during the therapeutic period (Cave) (i.e. Cmax/Cave≤10,preferably ≤8, more preferably ≤5).

SUMMARY OF THE INVENTION

The present invention provides a pharmaceutical formulation comprisingan appropriate combination of lipid excipients, organic alcoholicsolvent, polar solvent, peptide active agent comprising pasireotide andcertain optional components, that can be used as a depot-precursorformulation (referred to herein for brevity as a pre-formulation) toaddress one or more of the needs described above. The inventors haveestablished that by optimising these components, depot compositions ofpasireotide and corresponding precursor formulations with a highlyadvantageous combination of properties can be generated.

In a first aspect, the invention therefore provides a pre-formulationcomprising a low viscosity mixture of:

-   -   a. 20-50 wt. % of at least one diacyl glycerol;    -   b. 20-54 wt. % of at least one phosphatidyl choline (PC);    -   c. 5-15 wt. % of at least one biocompatible, organic        mono-alcoholic solvent;    -   d. 1 to 20 wt. % polar solvent    -   e. 5 to 150 mg/ml of at least one peptide somatostatin receptor        agonist comprising pasireotide (calculated as the free base);    -   f. optionally at least one antioxidant;        wherein the ratio of components a:b is in the range 40:60 to        54:46;        wherein the pre-formulation forms, or is capable of forming, at        least one liquid crystalline phase structure upon contact with        excess aqueous fluid.

Such compositions will preferably comprise glycerol dioleate (GDO), soyPC and/or high purity PC, (such as PC with at least 95% PC head groupsand at least 95% C16 to C20 acyl groups having 0 to 3 unsaturations),ethanol, water/propylene glycol and/or EDTA as components a), b), c), d)and f) respectively. Component e) comprises or consists of pasireotideor a salt thereof preferably wherein said salt is a biotolerable salt,such as one selected from the chloride, acetate, pamoate and tartratesalts, most preferably the pamoate salt, as described herein.

In a second embodiment, the invention correspondingly provides a processfor the formation of a pre-formulation suitable for the administrationof a peptide somatostatin receptor agonist comprising pasireotide to a(preferably mammalian) subject, said process comprising forming a lowviscosity mixture of:

-   -   a) 20-50 wt. % of at least one diacyl glycerol;    -   b) 20-54 wt. % of at least one phosphatidyl choline (PC);    -   c) 5-215 wt. % of at least one biocompatible, organic        mono-alcoholic solvent;    -   d) 1 to 20 wt. % polar solvent    -   e) 5 to 150 mg/ml of at least one peptide somatostatin receptor        agonist comprising pasireotide (calculated as the free base);    -   f) optionally at least one antioxidant;        wherein the ratio of components a:b is in the range 40:60 to        54:46;        and dissolving or dispersing the at least one peptide        somatostatin receptor agonist (preferably a somatostatin        analogue) in the low viscosity mixture, or in at least one of        components a), b), c), d) and optionally f) prior to forming the        low viscosity mixture. Such a pre-formulation will typically be        one as described herein.

The preformulations are highly useful for the controlled and sustainedrelease of peptide active, especially those requiring or benefiting froma very flat release profile and/or minimal “burst” upon administration.In a corresponding embodiment, the invention therefore provides for theuse of a low viscosity mixture of:

-   -   a) 20-50 wt. % of at least one diacyl glycerol;    -   b) 20-54 wt. % of at least one phosphatidyl choline (PC);    -   c) 5-20 wt. % of at least one biocompatible, organic        mono-alcoholic solvent;    -   d) 1 to 20 wt. % polar solvent    -   e) 5 to 150 mg/ml of at least one peptide somatostatin receptor        agonist comprising pasireotide (calculated as free base);    -   f) optionally at least one antioxidant;        wherein the ratio of components a:b is in the range 40:60 to        54:46;        in the manufacture of a pre-formulation for use in the sustained        administration of said peptide somatostatin receptor agonist.        Such a low viscosity mixture will preferably be one described        herein.

The peptide somatostatin receptor agonists in the formulations of thepresent invention are preferably pharmaceutically active. That is to saythat they provide a therapeutic, palliative and/or prophylactic effectwhen administered to a suitable subject (typically being one in need ofsuch an effect). In a further embodiment, the invention thereforeprovides a method for the treatment of a human or non-human mammaliansubject comprising administering to said subject a pre-formulation asdescribed herein.

Such a method may be for the treatment of a human or non-human mammaliansubject in need thereof to combat, (e.g. cure, treat, improve, prevent,palliate and/or ameliorate the symptoms of) at least one conditionselected from Cushing's disease, acromegaly, type I or type II diabetesmellitus, especially complications thereof, e.g. angiopathy, diabeticproliferative retinopathy, diabetic macular edema, nephropathy,neuropathy and dawn phenomenon, and other metabolic disorders related toinsulin or glucagon release, e.g. obesity, e.g. morbid obesity orhypothalamic or hyperinsulinemic obesity, enterocutaneous andpancreaticocutaneous fistula, irritable bowel syndrome, inflammatorydiseases, e.g. Grave's Disease, inflammatory bowel disease, psoriasis orrheumatoid arthritis, polycystic kidney disease, dumping syndrome,watery diarrhea syndrome, AIDS-related diarrhea, chemotherapy-induceddiarrhea, acute or chronic pancreatitis and gastrointestinal hormonesecreting tumors (e.g. GEP tumors, for example vipomas, glucagonomas,insulinomas, carcinoids and the like), lymphocyte malignancies, e.g.lymphomas or leukemias, hepatocellular carcinoma as well asgastrointestinal bleeding, e.g variceal oesophagial bleeding. Thepreformulations as described herein for use in such methods form afurther aspect of the invention.

Correspondingly, in a further aspect, the present invention provides theuse of a low viscosity mixture of:

-   -   a) 20-50 wt. % of at least one diacyl glycerol;    -   b) 20-54 wt. % of at least one phosphatidyl choline (PC);    -   c) 5-15 wt. % of at least one biocompatible, organic        mono-alcoholic solvent;    -   d) 1 to 20 wt. % polar solvent    -   e) 5 to 150 mg/ml of at least one peptide somatostatin receptor        agonist comprising pasireotide (calculated as free base);    -   f) optionally at least one antioxidant;        wherein the ratio of components a:b is in the range 40:60 to        54:46;        in the manufacture of a low viscosity pre-formulation medicament        for use in the in vivo formation of a depot for treatment of at        least one condition selected from Cushing's disease, acromegaly,        type I or type II diabetes mellitus, especially complications        thereof, e.g. angiopathy, diabetic proliferative retinopathy,        diabetic macular edema, nephropathy, neuropathy and dawn        phenomenon, and other metabolic disorders related to insulin or        glucagon release, e.g. obesity, e.g. morbid obesity or        hypothalamic or hyperinsulinemic obesity, enterocutaneous and        pancreaticocutaneous fistula, irritable bowel syndrome,        inflammatory diseases, e.g. Grave's Disease, inflammatory bowel        disease, psoriasis or rheumatoid arthritis, polycystic kidney        disease, dumping syndrome, watery diarrhea syndrome,        AIDS-related diarrhea, chemotherapy-induced diarrhea, acute or        chronic pancreatitis and gastrointestinal hormone secreting        tumors (e.g. GEP tumors, for example vipomas, glucagonomas,        insulinomas, carcinoids and the like), lymphocyte malignancies,        e.g. lymphomas or leukemias, hepatocellular carcinoma as well as        gastrointestinal bleeding, e.g variceal oesophagial bleeding.

In all appropriate aspects of the invention, the preferred conditionsare Cushing's disease and acromegaly.

One of the advantages of the formulations of the present invention overmany other controlled-release compositions is that they are stable tostorage in their final form and thus little or no preparation isrequired at the time of administration. This allows the pre-formulationsto be ready-to-administer and also to be supplied in convenient,ready-to-administer form. In a further aspect, the invention thereforeprovides a pre-filled administration device containing a pre-formulationas described herein. Such a device will generally provide either asingle administration or multiple administrations of a composition whichwill deliver, for example, a dosage of somatostatin receptor agonist inthe range of 0.2 to 3 mg/day pasireotide.

In a further aspect the invention provides a kit comprising saidadministration device according to the invention.

The kit can optionally contain instructions for subcutaneous orintramuscular administration of said composition. All compositionsdescribed herein are suitable for use in such a kit and may thus becontained therein.

The kits of the invention can optionally include additionaladministration components such as needles, swabs, and the like and willoptionally contain instructions for administration.

BRIEF SUMMARY OF THE ATTACHED FIGURES

FIG. 1 . Flow chart describing the preparation of lipid/SOM230(pasireotide) samples for solubility screening.

FIG. 2 . Injectability (seconds/mL) measured at 20N constant force as afunction of formulation composition and using 1 mL long Luer-Lock glasssyringe with 23G ⅝″ (16 mm) needle. The formulations numbers refer tothe sample ID (last two digits) in Table 3. The data points representthe mean of duplicate measurements.

FIG. 3 . Injectability (seconds per mL), measured at 20N constant force,as a function of formulation viscosity and using the indicated syringeand needle configuration.

FIG. 4 . Comparison of stability data on lipid/pasireotide formulationsdifferentiated by their respective solvent composition (SPC/GDO weightratio constant at 50/50), with three different SOM230 (pasireotide) saltforms (pamoate (Pm), acetate (Ac) and hydrochloride (Cl)) after storagefor 2 weeks at 60° C. The pasireotide free base concentration at startwas in all cases approximately 30 mg/mL.

FIG. 5 . Mean plasma concentrations of SOM230 (pasireotide pamoate)after s.c. injection in rats. The error bars denote standard deviation(n=6). Data obtained in PK-12-437.

FIG. 6 . Mean plasma concentrations of SOM230 (pasireotide pamoate)after s.c. injection in rats. The error bars denote standard deviation(n=6). Data obtained in PK-12-438.

FIG. 7 . Dose linearity with respect to exposure (AUC) in studyPK-12-438. Error bars represent standard deviation.

FIG. 8 . Dose linearity with respect to Cmax in study PK-12-438. Errorbars represent standard deviation.

FIG. 9 . Mean plasma concentrations of SOM230 (pasireotide pamoate)after s.c. injection in rats. The error bars denote standard deviation(n=6). Data obtained in PK-12-451.

FIG. 10 . The SOM230 purity after storage at 5° C. The figure legendrefers to the respective batch number as indicated in Table 19.

FIG. 11 . The SOM230 purity after storage at 25° C./60% RH. The figurelegend refers to the respective batch number as indicated in Table 19.

DETAILED DESCRIPTION OF THE INVENTION

The formulations of the present invention generate a non-lamellar liquidcrystalline phase following administration. The use of non-lamellarphase structures (such as liquid crystalline phases) in the delivery ofbioactive agents is now relatively well established. A most effectivelipid depot system for general use is described in WO2005/117830, and asuitable lipid matrix for use in the present invention is described ingeneral terms in that document, the full disclosure of which is herebyincorporated herein by reference. For a description of the mostfavourable phase structures of such formulations, attention is drawn tothe discussion in WO2005/117830 and particularly to page 29 thereof.

All % are specified by weight herein throughout, unless otherwiseindicated. Furthermore, the % by weight indicated is the % of the totalpre-formulation including all of the components indicated herein wherecontext allows. Weight percentages of pasireotide will be calculated onthe basis of the weight of free acid irrespective of whether the acid ora salt thereof is used. The pre-formulations can optionally consist ofessentially only the components indicated herein (including whereappropriate additional optional components indicated herein below and inthe attached claims) and in one aspect consist entirely of suchcomponents. Where a formulation is indicated as “consisting essentiallyof” certain components herein, when the specified components provide theessential nature of that formulation, such as when the specifiedcomponents constitute at least 95%, preferably at least 98%, of theformulation.

The lipid-based systems described herein comprise lipid components a)and b), plus organic mono-alcoholic solvent (c), polar solvent (d),peptide somatostatin receptor agonist comprising pasireotide (e) andoptional antioxidant (f) components.

Preferably the pre-formulation according to the invention is a molecularsolution or has an L₂ phase structure (prior to administration).Preferably the pre-formulation forms a non-lamellar (e.g. liquidcrystalline) phase following administration. Such a phase change istypically brought about by absorption of aqueous fluid from thephysiological environment, as indicated herein.

The present inventors have now surprisingly established that byappropriate choice of types, absolute amounts and ratios of lipidcomponents along with a peptide somatostatin receptor agonist comprisingpasireotide and at least two solvents including an alcohol and at leastone polar solvent, the release properties of the depot compositionsformed from the pre-formulations of the invention can be rendered highlyadvantageous. In particular, by using a mixture of an alcohol and apolar solvent (especially at the weight ratios close to 1:1 describedherein (e.g. between 10:1-1:3, preferably 5:1-1:2 and most preferably2:1-2:3)) the advantages of the alcohol solvent on the release profilecan be maintained while other properties such as the comfort onadministration and/or the viscosity of the formulation can be improved.Alternatively or in addition to this, the release profile of thesomatostatin receptor agonist can be made remarkably level, with themaximum plasma concentration in vivo being only a small multiple of theaverage or even minimum concentration during the dosing period. Suchadvantages apply even in comparison with other lipid depot compositions,which in themselves offer previously unobtainable standards incontrolled release.

It is important, particularly with certain peptide active agents, suchas somatostatin analogues (e.g. pasireotide), to control the peakconcentration (Cmax) of drug in the plasma to a level equal to or lessthan that tolerable to the subject, for example to avoid side-effectssuch as flushing or severe nausea, while providing or achieving atherapeutically effective level over the desired period of release.Generally, the average concentration during the period of release beforethe next dose is administered, Cave, falls within the therapeutic range.Control over the maximal (Cmax) and minimum (Cmin) concentrations isalso important in order to achieve the desired treatment over time. Inone embodiment, the initial burst (e.g. during the first 12 hoursfollowing administration) is not the Cmax of the release profile.

Whether or not the initial burst is also the Cmax, preferably theCmax/Cave ratio is less than 50, preferably less than or equal to 15,more preferably less than or equal to 10, even more preferably less thanor equal to 5. Furthermore, it is preferred that the Cmax/Cmin ratio isnot more than 50, preferably less than or equal to 15, more preferablyless than or equal to 10, even more preferably less than or equal to 5.Cmax is defined as is known in the art, as the peak or maximal plasmaconcentration observed during the period of release before the next doseis administered and Cave is defined as the average plasma concentrationduring that period of release. Cmin is correspondingly the minimalconcentration during that period. Cave can be calculated by calculatingthe drug present in the plasma as area under the curve (AUC) over theselected period of time, generally the entire period of release beforethe administration of the next dose, and dividing by that period oftime.

Component a)—Diacyl Glycerol

Preferable ranges for component a) are 20-80 wt. %, preferably 30-70 wt.%, more preferably 20-50%, such as 33-60% (e.g. 43-60%, 30 to 43% or30-40%), particularly 38 to 43%, around 32% (e.g. ±2) and/or around 40%(e.g. ±2). Preferable ranges of component b) are 20-80 wt. %, preferably30-70 wt. % (e.g. 30-45%), more preferably 33-55% (e.g. 35-55%),particularly 38 to 43%.

Ratios of a:b are typically 40:60 to 70:30, preferably 45:55 to 55:45and more preferably 40:60 to 54:46 or 42:58 to 48:52. Ratios of around50:50 (e.g. ±2) and around 45:55 (e.g. ±3 a:b) are highly effective.

Component “a” as indicated herein is preferably at least one diacylglycerol (DAG) and thus has two non-polar “tail” groups. The twonon-polar groups may have the same or a differing number of carbon atomsand may each independently be saturated or unsaturated. Examples ofnon-polar groups include C₆-C₃₂ alkyl and alkenyl groups, which aretypically present as the esters of long chain carboxylic acids. Theseare often described by reference to the number of carbon atoms and thenumber of unsaturations in the carbon chain. Thus, CX:Z indicates ahydrocarbon chain having X carbon atoms and Z unsaturations. Examplesparticularly include lauroyl (C12:0), myristoyl (C14:0), palmitoyl(C16:0), phytanoyl (C16:0), palmitoleoyl (C16:1), stearoyl (C18:0),oleoyl (C18:1), elaidoyl (C18:1), linoleoyl (C18:2), linolenoyl (C18:3),arachidonoyl (C20:4), behenoyl (C22:0) and lignoceroyl (C24:9) groups.Thus, typical non-polar chains are based on the fatty acids of naturalester lipids, including caproic, caprylic, capric, lauric, myristic,palmitic, phytanic, palmitolic, stearic, oleic, elaidic, linoleic,linolenic, arachidonic, behenic or lignoceric acids, or thecorresponding alcohols. Preferable non-polar chains are palmitic,stearic, oleic and linoleic acids, particularly oleic acid.

Mixtures of any number of diacyl lipids may be used as component a).Preferably this component will include at least a portion of C18 lipids(e.g. DAG having one or more (i.e. one or two) C18:0, C18:1, C18:2 orC18:3 non-polar groups), such as glycerol dioleate (GDO) and/or glyceroldilinoleate (GDL). A highly preferred example is DAG comprising at least50%, preferably at least 80% and even comprising substantially 100% GDO.

Since GDO and other diacyl glycerols are products derived from naturalsources, there is generally a certain proportion of “contaminant” lipidhaving other chain lengths etc. In one aspect, GDO as used herein isthus used to indicate any commercial grade of GDO with concomitantimpurities (i.e. GDO of commercial purity). These impurities may beseparated and removed by purification but providing the grade isconsistent this is rarely necessary. If necessary, however, “GDO” may beessentially chemically pure GDO, such as at least 80% pure, preferablyat least 85% pure and more preferably at least 90% pure GDO.

Component b)—Phosphatidyl Choline

Component “b” in the preferred lipid matrices of the present inventionis at least one phosphatidyl choline (PC). As with component a), thiscomponent comprises a polar head group and at least one non-polar tailgroup. The difference between components a) and b) lies principally inthe polar group. The non-polar portions may thus suitably be derivedfrom the fatty acids or corresponding alcohols considered above forcomponent a. As with component a), the PC will contain two non-polargroups. Again, C18 groups are preferred and may be combined with anyother suitable non-polar group, particularly C16 groups.

The phosphatidyl choline portion, even more suitably than any diacylglycerol portion, may be derived from a natural source. Suitable sourcesof phospholipids include egg, heart (e.g. bovine), brain, liver (e.g.bovine) and plant sources including soybean. Such sources may provideone or more constituents of component b, which may comprise any mixtureof phospholipids. Any single PC or mixture of PCs from these or othersources may be used, but mixtures comprising soy PC or egg PC are highlysuitable. The PC component preferably contains at least 50% soy PC oregg PC, more preferably at least 75% soy PC or egg PC and mostpreferably essentially pure soy PC or egg PC.

In one embodiment applicable to all aspects of the invention, componentb) comprises PC. Preferably the PC is derived from soy. Preferably thePC comprises 18:2 fatty acids as the primary fatty acid component with16:0 and/or 18:1 as the secondary fatty acid components. These arepreferably present in the PC at a ratio of between 1.5:1 and 6:1. PChaving approximately 60-65% 18:2, 10 to 20% 16:0, 5-15% 18:1, with thebalance predominantly other 16 carbon and 18 carbon fatty acids ispreferred and is typical of soy PC.

In an alternative but equally preferred embodiment, also applicable toall aspects of the invention, the PC component may comprise syntheticdioleoyl PC. This is believed to provide increased stability and so willbe particularly preferable for compositions needing to be stable to longterm storage, and/or having a long release period in vivo. In thisembodiment the PC component preferably contains at least 50% syntheticdioleoyl PC, more preferably at least 75% synthetic dioleoyl PC and mostpreferably essentially pure synthetic dioleoyl PC. Any remaining PC ispreferably soy or egg PC as above.

In one embodiment, the precursor formulations of the present inventionare comprised at least partially of synthetic DOPC (i.e. PC having atleast 95% PC head groups and at least 90% oleoyl acyl groups) and has astability to storage at 15-25° C., defined as less than 5% peptidedegradation as assayed by peptide purity, of at least 6 months, morepreferably at least 12 months and most preferably at least 24 months.

Since the pre-formulations of the invention are to be administered to asubject for the controlled release of a peptide active agent, it isimportant that the components are biocompatible. In this regard, thepreferred lipid matrices for use in the pre-formulations of the presentinvention are highly advantageous since both PC and DAGs are welltolerated and are broken down in vivo into components that are naturallypresent in the mammalian body.

Synthetic or highly purified PCs, such as dioleoyl phosphatidylcholine(DOPC) and palmitoyl oleoyl phosphatidylcholine (POPC), as well as theother various high-purity PCs described herein, are highly appropriateas all or part of component b).

In a highly preferred embodiment, component b) is a “high purity” PC asfollows: b. at least one phospholipid component comprising phospholipidshaving

-   -   i. polar head groups comprising at least 95% phosphatidyl        choline, and    -   ii. two acyl chains each independently having 16 to 20 carbons        wherein at least one acyl chain has at least one unsaturation in        the carbon chain, and there are no more than four unsaturations        over two carbon chains;

Typically, this may be PC with at least 95% PC head groups and at least95% C16 to C20 acyl chains having 0 to 3 unsaturations.

The synthetic dioleoyl PC is most preferably1,2-dioleoyl-sn-glycero-3-phosphocholine, and other synthetic PCcomponents include DDPC (1,2-Didecanoyl-sn-glycero-3-phosphocholine);DEPC(1,2-Dierucoyl-sn-glycero-3-phosphocholine);DLOPC(1,2-Dilinoleoyl-sn-glycero-3-phosphocholine);DLPC(1,2-Dilauroyl-sn-glycero-3-phosphocholine);DMPC(1,2-Dimyristoyl-sn-glycero-3-phosphocholine);DOPC(1,2-Dioleoyl-sn-glycero-3-phosphocholine);DPPC(1,2-Dipalmitoyl-sn-glycero-3-phosphocholine);DSPC(1,2-Distearoyl-sn-glycero-3-phosphocholine);MPPC(1-Myristoyl-2-palmitoyl-sn-glycero 3-phosphocholine);MSPC(1-Myristoyl-2-stearoyl-sn-glycero-3-phosphocholine);PMPC(1-Palmitoyl-2-myristoyl-sn-glycero-3phosphocholine);POPC(1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine);PSPC(1-Palmitoyl-2-stearoyl-sn-glycero-3-phosphocholine);SMPC(1-Stearoyl-2-myristoyl-sn-glycero-3-phosphocholine);SOPC(1-Stearoyl-2-oleoyl-sn-glycero-3-phosphocholine); andSPPC(1-Stearoyl-2-palmitoyl-sn-glycero-3-phosphocholine), or anycombination thereof.

In some circumstances, such as the absence of preserving agents such asEDTA, the use of synthetic or highly purified PCs (e.g. DOPC) mayprovide greater stability for the somatostatin receptor agonist in theformulations. Thus in one embodiment, component b) may comprise (e.g.may comprise at least 75%) synthetic or highly purified (e.g.purity >90%) PCs (e.g. DOPC). This may particularly be in the absence ofchelating agents such as EDTA. In an alternative embodiment, componentb) may comprise (e.g. comprise at least 75%) naturally derived PCs, suchas soy PC or egg PC. This will particularly be where at least onestabilising component (such as an antioxidant, chelator etc) is includedin the precursor formulation.

A particularly favoured combination of components a) and b) are GDO withPC, especially GDO with soy PC and/or “high purity” PC. Appropriateamounts of each component suitable for the combination are those amountsindicated herein for the individual components in any combination. Thisapplies also to any combinations of components indicated herein, wherecontext allows.

The ratio of components a:b is in the range 40:60 to 54:46. Preferablythe a:b ratio is in the range 45:55 to 54:46, more preferably 47:53 to53:47. Most preferably the a:b ratio is approximately 50:50.

In one embodiment applicable to all aspects of the invention, it ispreferred if the a:b ratio is in the range 40:60 to 49:51. In analternative embodiment, the ratio may be in the range 42:58 to 52:48.

Component c)—Organic Mono-Alcoholic Solvent

Component c) of the pre-formulations of the invention is an organicmono-alcoholic solvent. Since the pre-formulation is to generate a depotcomposition following administration (e.g. in vivo), typically uponcontact with excess aqueous fluid, it is desirable that this solvent betolerable to the subject and be capable of mixing with the aqueousfluid, and/or diffusing or dissolving out of the pre-formulation intothe aqueous fluid. Solvents having at least moderate water solubilityare thus preferred.

Most preferably component c) comprises or consists of ethanol, propanol,ispropanol, benzyl alcohol or mixtures thereof. Most preferablycomponent c) comprises or consists of ethanol.

In a preferred embodiment, the solvent is such that a relatively smalladdition to a mixture comprising a) and b) (i.e. preferably below 15%)gives large viscosity reductions, of one order of magnitude or more. Asdescribed herein, the addition of 10% organic mono-alcohol solvent cangive a reduction of two or more orders of magnitude in viscosity overthe solvent-free composition, or over a depot containing only a polarsolvent such as water, or glycerol.

The amount of component c) in the pre-formulation will have aconsiderable effect upon several features. In particular, the viscosityand the rate (and duration) of release will alter significantly with thesolvent level. The amount of solvent will thus be at least sufficient toprovide a low viscosity mixture but will additionally be determined soas to provide the desired release rate. This may be determined byroutine methods in view of the Examples below. Typically a level of 0.1to 35%, particularly 5 to 25% solvent will provide suitable release andviscosity properties.

This will preferably be 5 to 16% (e.g. 6 to 14%) and an amount of around8% (e.g. 8±2%) is highly effective.

As indicated above, the amount of component c) in the pre-formulationsof the invention will be at least sufficient to provide a low viscositymixture (e.g. a molecular solution, see above) of components a), b), c)and d) and optionally f) and will be easily determined for anyparticular combination of components by standard methods.

The phase behaviour may be analysed by techniques such as visualobservation in combination with polarized light microscopy, X-rayscattering and diffraction techniques, nuclear magnetic resonance, andcryo-transmission electron microscopy (cryo-TEM) to look for solutions,L₂ or L₃ phases, or liquid crystalline phases or as in the case ofcryoTEM, dispersed fragments of such phases. Viscosity may be measureddirectly by standard means. As described above, an appropriate practicalviscosity is that which can effectively be syringed and particularlysterile filtered. This will be assessed easily as indicated herein.

Typical organic mono-alcoholic solvents suitable for use in theinvention include at least one solvent selected from ethanol, propanol,isopropanol, and benzyl alcohol, particularly ethanol.

A highly preferred combination for components a), b) and c) is soy PCand/or “high purity PC”, GDO and ethanol. As indicated above,appropriate amounts of each component suitable for the combination arethose amounts indicated herein for the individual components, in anycombination.

It is preferable that little or none of component c) contains halogensubstituted hydrocarbons since these tend to have lowerbiocompatibility. For example, the content of halogenated organicsolvents may be less than 0.5%, preferably less than 0.1%.

Component c) as used herein may be a single solvent or a mixture ofsuitable solvents but will generally be of low viscosity. This isimportant because one of the key aspects of the present invention isthat it provides pre-formulations that are of low viscosity and aprimary role of a suitable solvent is to reduce this viscosity.

This reduction will be a combination of the effect of the lowerviscosity of the solvent and the effect of the molecular interactionsbetween solvent and lipid composition. One observation of the presentinventors is that the oxygen-containing solvents of low viscositydescribed herein have highly advantageous and unexpected molecularinteractions with the lipid parts of the composition, thereby providinga non-linear reduction in viscosity with the addition of a small volumeof solvent.

The viscosity of the “low viscosity” solvent component c) (singlesolvent or mixture) should typically be no more than 18 mPas at 20° C.This is preferably no more than 15 mPas, more preferably no more than 10mPas and most preferably no more than 7 mPas at 20° C.

Component d)—Polar Solvent

Some of the particular benefits of the compositions of the presentinvention come through the unexpected finding that the use of an alcoholsolvent in combination with a polar solvent such as a diol or waterallows a significant improvement in the performance of certainlipid-based controlled-release compositions. In particular, the additionof a diol (such as propylene glycol) or water has been observed to allowthe proportion of alcohol to be increased without adversely affectingthe release profile and/or allow an improvement in the release profileand/or allow higher loading of the somatostatin receptor agonist. By“adversely affecting the release profile” is intended to indicate thatthe ratio of Cmax/Cave is increased and/or the ratio of Cmax/Cmin isincreased (for example increased by a factor of at least 1.2). Similarlyan improvement in the release profile indicates that the ratio ofCmax/Cave and/or Cmax/Cmin is decreased (e.g. decreased by a factor ofat least 1.2.).

Typical polar solvents will have a comparatively high dielectricconstant corresponding to their high polarity. Thus, suitable polarsolvents will generally have a dielectric constant of at least 28 at 25°C., more preferably at least 30 at 25° C. Highly suitable examplesinclude water (˜80), propylene glycol (˜32) and N-methyl-2-pyrrolidone(NMP, ˜32).

Although it has previously been suggested that lipid controlled-releasecompositions should be formulated substantially in the absence of water,in order to avoid the conversion to high-viscosity liquid crystallinephases, it has now furthermore been established that a small andcarefully controlled amount of a polar solvent such as water can provideconsiderable benefits. In particular, the inclusion of this polarsolvent (preferably comprising water) allows further improvements incontrolling the initial release of somatostatin receptor agonist, allowshigher stable loading of some peptide somatostatin receptor agonists,provides faster depot formation and/or provides further reduceddiscomfort upon injection. Any one of these factors potentially providesa significant improvement in the context of therapeutic drug delivery,patient health and/or patient compliance.

The pre-formulations of the present invention must thus also contain apolar solvent, component d). A suitable amount will typically be greaterthan 1% by weight of the pre-formulation, for example 1-30 wt. %,particularly 1.2-20 wt. %, especially 2-18 wt. %. More preferablycomponent d) is present in the range 5-15 wt. %, especially 6-12 wt. %.Component d) is preferably water, propylene glycol or mixtures thereof.In one preferred aspect, the pre-formulations of the invention containethanol as component c) with water and/or propylene glycol as componentd).

In one embodiment the pre-formulation comprises at least 1.5% (e.g. atleast 4.5%) water as part of component d) (by weight of the totalcomposition) with the remainder being propylene glycol. At least 5%water with the balance of component d) being PG is preferred. Componentd) may comprise or consist of water.

In an alternative embodiment, component d) may comprise or consist ofpropylene glycol.

Preferably the total level of components c) and d) is not more than 35wt. %, preferably not more than 30 wt. %, more preferably not more than25 wt. %, most preferably not more than 20 wt. %. For example the totallevel of components c) and d) may be in the range 10-30 wt. %,preferably 12-25 wt. %, most preferably 15-20 wt. %.

The ratio of components c) and d) will also have potential advantages inthe compositions of the invention. In particular, by inclusion of somepolar solvent which is miscible with the mono-alcohol component(especially water), the slight sensation that may be caused at theinjection site from the alcohol content can be substantially eliminated.Thus, in one embodiment, the ratio of components c):d) may be in therange 30:70 to 70:30, more preferably 40:60 to 60:40. In one embodiment,the amount of alcohol component c) by weight is no greater than theamount of polar solvent d). Ratios of c):d) ranging from 30:70 to 50:50are thus appropriate in such an embodiment. Approximately equal amountsof components c) and d) are highly appropriate.

A highly preferred combination for the lipid matrix aspect is soy PCand/or a C16 to C20 PC of at least 95% purity such as DOPC (as describedherein), with GDO, ethanol, and water/propylene glycol or mixturesthereof. The solvent may be, for example, ethanol and water in theabsence of PG, ethanol and PG in the absence of water, or a mixture ofall three. As indicated above, appropriate amounts of each componentsuitable for the combination are those amounts indicated herein for theindividual components, in any combination.

Component e)—Peptide Active Agent (Somatostatin Receptor Agonist)

The pre-formulations of the present invention contain at least onepeptide somatostatin receptor agonist comprising pasireotide. Suitablepeptides for use in the necessary peptide somatostatin receptor agonistsmay be naturally occurring or derived from natural peptides, or may bechemically modified or wholly synthetic peptide molecules. Any aminoacids may be comprised in the peptides including those described herein,and the peptides may be chemically or enzymatically modified. Similarly,peptide somatostatin receptor agonists may be linear or cyclised bymeans of one or more covalent or non-covalent interactions. Pasireotide,for example, comprises a cyclic portion of six peptide residues (seebelow).

Typical peptide actives will be in the range of 500 to 100,000 amu inmolecular weight and can evidently include protein somatostatin receptoragonists. In one embodiment, the polypeptides can have at least onecationic charge at neutral and/or physiological pH, and most preferablywill require at least one anionic counter-ion at pH 6.5 or above,preferably at pH 7.5 or above. This counter-ion will be physiologicallyacceptable, and may thus be a halide or the ion of a physiologicallyacceptable acid. Acetate, pamoate and tartrate counter ions and/orchloride ions are particularly preferred and therefore in one embodimentof the invention, the somatostatin receptor agonist is pasireotidepamoate.

In particular, the present inventors have surprisingly established thatpasireotide pamoate is surprisingly more stable to storage whenformulated with the lipid excipients described herein than is thecorresponding acetate or chloride salt. This is particularly surprisingsince the acetate is the most commonly used form of many small peptideactive agents, such as the somatostatin analogue octreotide.Furthermore, the chloride salt has been shown in previous work to besurprisingly effective in formulations of certain actives, such asoctreotide. However, in the present case, storage at 60° C. informulations of the present invention illustrated a markedly higherstability for the pamoate over the acetate and even the chloride ofpasireotide (see examples and Figures below). The pamoate salt is thusthe preferred form of the somatostatin receptor agonist comprisingpasireotide.

In the peptide actives of the present invention, peptides may containonly amino acids selected from those 20 α-amino acids indicated in thegenetic code, or more preferably may contain their isomers and othernatural and non-natural amino acids, (generally α, β or γ amino acids)and their analogues and derivatives.

Amino acid derivatives are especially useful at the termini of thepeptides, where the terminal amino or carboxylate group may besubstituted by or with any other functional group such as hydroxy,alkoxy, carboxy (on the N-terminal end), ester, amide, thio, amido,amino (on the C-terminal end), alkyl amino, di- or tri-alkyl amino,alkyl (by which is meant, herein throughout C₁-C₂₀ alkyl, preferablyC₁-C₁₈ alkyl e.g. methyl, ethyl, n-propyl, isopropyl, n-butyl, iso-,sec- or t-butyl etc.), aryl (e.g phenyl, benzyl, napthyl etc),heteroaryl, or other functional groups, preferably with at least oneheteroatom and preferably having no more than 20 atoms in total, morepreferably no more than 10 and most preferably not more than 6 atoms(optionally excluding hydrogens).

In the present invention, the peptide somatostatin receptor agonistcomprises pasireotide, which is a somatostatin analogue. Thesomatostatin receptor agonist may also comprise other peptides such asother peptide analogues of somatostatin and may include octreotide,somatostatin 14 and/or somatostatin 28.

Somatostatin has two active forms produced by alternative cleavage of asingle pre-protein: one of 14 amino acids, the other of 28 amino acids.Somatostatin 1-14 is a cyclic peptide hormone having the sequenceAla-Gly-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys, where the twocystine residues are connected by a disulphide bridge to generate a typeII β-turn at the key binding sequence of Phe-Trp-Lys-Thr. Somatostatinis a natural peptide hormone also known as Growth Hormone ReleaseInhibiting Factor and has a role as an antagonist of insulin, glucogenand certain other hormones in the release of somatotrophin (Human GrowthHormone). The biological half-life of natural Somatostatin is very short(1-3 minutes) and so in itself is difficult to formulate as a viabletherapeutic. However, the lipid depot compositions of the presentinvention are highly effective for short-lived active agents and anincreasing number of somatostatin analogues are becoming available withhigher activities and/or longer clearance times in vivo. Pasireotide isone such analogue and forms the essential peptide active agent of thecompositions of the present invention.

Somatostatin analogues, including pasireotide, but also such asoctreotide, lanreotide, vapreotide and related peptides, are used orindicated in the treatment of a variety of conditions where they aretypically administered over an extended period. In one embodiment of theinvention, the peptide active agent comprises or consists of pasireotideas well as another somatostatin analogue selected from the groupconsisting of octreotide, lanreotide and vapreotide. In one embodiment,the peptide active agent of the invention comprises or consists ofpasireotide and octreotide. In a further embodiment, pasireotide mayform the sole active agent.

Octreotide, for example, is the synthetic octa-peptide with sequenceD-Phe-Cys-Phe-D-Trp-Lys-Thr-Cys-Thr-ol (2-7 disulphide bridge) and istypically administered as the acetate salt. Several clinical studiesalso feature the octreotide pamoate. This derivative retains the keyPhe-(D)Trp-Lys-Thr β-turn but, in contrast to the natural hormone, has aterminal half-life of around 1.7 hours. Octreotide is used in treatmentof conditions including carcinoid tumours and acromegaly, and after aninitial dose is typically given over a sustained period of weeks, ormore commonly many months or years. In addition, somatostatin analoguesare indicated in the treatment of many cancers since a wide variety oftumours are found to express somatostatin receptors. Of particularinterest are those expressing the “sst(2)” and/or “sst(5)” receptor.

As used herein, the term “somatostatin receptor agonist” is used toindicate a compound having an agonistic function at one or moresomatostatin receptors (SSTRs). There are five known types of SSTRs(SSTR1-SSTR5), showing equally high affinity for SST-14. The mostinvestigated somatostatin receptor agonists, including octreotide, showhigh selectivity for SSTR2 and SSTR5. Thus in one preferred embodiment,somatostatin receptor agonists as indicated herein have an agonisticfunction at somatostatin receptors including SSTR2 and/or SSTR5.

The most common “simple” formulation of Octreotide is “Sandostatin”®from Novartis. This is a solution for subcutaneous (s.c) injection and a100 μg dose reaches a peak concentration of 5.2 ng/ml at 0.4 hours postinjection. The duration of action can be up to 12 hours but s.c. dosingis generally carried out every 8 hours. Evidently, s.c. injection 3times daily for periods of months or years is not an ideal dosingregime.

In order to avoid the need for multiple daily injections of octreotide,a further formulation is available; “Sandostatin LAR”®, again fromNovartis. This is a formulation of octreotide in poly lactic co-glycolicacid microspheres which, after reconstitution in an aqueous diluent, maybe administered by intra muscular (i.m.) injection.

Pasireotide is a multireceptor-targeted somatostatin analogue with highaffinity for somatostatin receptor subtypes sstr1,2,3 and sstr5 that hasbeen developed for the treatment of neuroendocrine diseases. Twoformulations of pasireotide have currently been developed: animmediate-release formulation for subcutaneous (sc) injection and along-acting-release (LAR) formulation. The structure of pasireotide isas follows:

Pasireotide was initially developed by Novartis Pharma as a treatmentfor Cushing's disease/syndrome and acromegaly, but has potentialapplicability in the treatment of several conditions for whichsomatostatin analogues such as octreotide are indicated, includingcarcinoid tumours.

Following a single subcutaneous dose of pasireotide, human plasma levelstypically peak quickly, at around 15 minutes to 1 hour after dosing,with an initial half-life of 2-3 hours following that peak. Althoughclearance half-life is greater for later phases of the decline, it isclear that the Cmax/Cave for such a delivery will be rather high.

Pasireotide LAR is a long acting formulation of pasireotide whichaddresses some of the above issues. However, this is a polymermicroparticle based system with the inherent limitations of such asystem, as are known in the art and described herein above.

Carcinoid tumours are intestinal tumour arising from specialised cellswith paracrine functions (APUD cells). The primary tumour is commonly inthe appendix, where it is clinically benign. Secondary, metastatic,intestinal carcinoid tumours secrete excessive amounts of vasoactivesubstances, including serotonin, bradykinin, histamine, prostaglandins,and polypeptide hormones. The clinical result is carcinoid syndrome (asyndrome of episodic cutaneous flushing, cyanosis, abdominal cramps, anddiarrhea in a patient with valvular heart disease and, less commonly,asthma and arthropathy). These tumours may grow anywhere in thegastrointestinal tract (and in the lungs) with approximately 90% in theappendix. The remainder occurs in the ileum, stomach, colon or rectum.Currently, treatment of carcinoid syndrome starts with i.v. bolusinjection followed by i.v. infusion. When sufficient effect on symptomshas been established, treatment with a depot formulation of octreotideformulated in poly lactic-co-glycolic acid (PLGA) microspheres isstarted. However, during the first two weeks or more after injection ofthe depot, daily s.c. injections with octreotide are recommended tocompensate for the slow release from the PLGA spheres.

Acromegaly is a rare chronic and insidious hormonal disorder that occurswhen the pituitary gland produces excess growth hormone (GH). It mostcommonly affects middle-aged adults and may lead to premature death.

Diabetes mellitus, hypertension, and increased risk of cardiovasculardisease are the most serious health consequences of acromegaly. Inaddition, patients with acromegaly are at an increased risk ofdeveloping colon polyps, which can become cancerous. The prevalence ofacromegaly is approximately 60 cases per million population, and theincidence is 3.3 new cases per million per year. The word acromegalycomes from the Greek words for “extremities” (acro) and “great”(megaly), because one of the most common symptoms of this condition isabnormal growth of the hands and feet.

Acromegaly is caused by prolonged overproduction of growth hormone (GH)and excessive production of insulin-like growth factor-I (IGF-I). In 98percent of cases, the overproduction of GH is caused by a pituitaryadenoma. The rate of GH production and the aggressiveness of the tumourvary from patient to patient. Generally, more aggressive tumours areseen in younger patients.

Acromegaly is a severe disease often diagnosed late. Morbidity andmortality rates are high, in particular, because of associatedcardiovascular, cerebrovascular, and respiratory disorders andmalignancies.

Current treatment of acromegaly is typically initiated by a period ofs.c. injections three times per day (optimal daily dose=300 μgoctreotide). After the last s.c. dose and providing a suitable effect isobserved then treatment with a depot formulation of octreotideformulated in poly lactic-co-glycolic acid (PLGA) microspheres isstarted. Dose adjustments are made after measurement of biomarkers (HGand IGF-1), typically after around 3 months.

The existing octreotide slow release formulation relies upon awell-established degrading-polymer type of depot formulation. Typicallysuch formulations are based on a biodegradable polymer such poly (lacticacid) (PLA) and/or poly (lactic-co-glycolic acid) (PLGA) and may be inthe form of a solution in an organic solvent, a pre-polymer mixed withan initiator, encapsulated polymer particles or (as in the case ofoctreotide) polymer microspheres.

In one typical embodiment, the peptide somatostatin receptor agonistcomprising pasireotide will generally be formulated as 0.02 to 12% byweight of the total formulation. Typical values will be 0.1 to 10%,preferably 0.2 to 8%, more preferably 0.5 to 6% (e.g. 1 to 3%). Theselevels may be applied to all aspects of the invention, where contextallows. A further preferred range is between 0.5 to 4 wt. %, morepreferably 1-3 wt. %, and most preferably 1.5-2.5 wt. %.

In one embodiment of the present invention, the concentration ofPasireotide in the precursor formulations of the present invention isgreater than 4% and the ratio of components a)/b) is less than 1. Thatis to say the weight percentage of component a) is less than that forcomponent b). In particular, in this embodiment of high Pasireotidecontent, the ratio of a) to b) may be between 49:51 and 40:60,preferably 48:52 to 42:58.

In a related embodiment, the peptide somatostatin receptor agonist maybe formulated at a level which cannot easily be achieved in the absenceof the polar solvent component of the mixture. In such an embodiment,the pasireotide content is typically at least 0.7%, preferably at least1%, more preferably at least 1.8% or at least 2% by weight offormulation. Levels of at least 3% and at least 4% are achievable withthe present invention, as are loading levels up to 8, 10 or 12%. Suchcompositions of the present invention typically not only contain a veryhigh level of peptide somatostatin receptor agonist as indicated, butare additionally stable to storage with no or low degradation of thesomatostatin receptor agonist (e.g. less than 5%) for considerableperiods, as indicated herein. Such periods will generally be at least amonth at 25° C. or at least a month at 5° C., preferably at least 3months, more preferably at least 6 months, most preferably 12 to 24months at 5° C. or alternatively at 25° C. These degrees of stabilityare applicable to all aspects of the invention, where context allows andrelate to stability both of the somatostatin receptor agonist and of thephase behaviour of the pre-formulation.

In a related embodiment, in the situation where a peptide somatostatinreceptor agonist is highly soluble in the alcohol component, it may bean advantage to limit this solubility of this agent. Without being boundby theory, it is thought that excessive solubility in this alcoholcomponent may result in the alcohol transporting a significant quantityof somatostatin receptor agonist out of the depot composition as itforms in vivo. Therefore, in one embodiment of the present invention,the polar solvent is used to control the solubility of the somatostatinreceptor agonist in the pre-formulation so as to aid control of therelease profile.

In a further aspect, the present invention therefore provides a methodfor controlling the solubility of a peptide somatostatin receptoragonist comprising pasireotide in a low viscosity mixture comprising:

-   -   a) 20-50 wt. % of at least one diacyl glycerol;    -   b) 20-54 wt. % of at least one phosphatidyl choline (PC);    -   c) 5-15 wt. % of at least one biocompatible, organic        mono-alcoholic solvent;    -   e) 5 to 150 mg/ml of at least one peptide somatostatin receptor        agonist comprising pasireotide;    -   f) optionally at least one antioxidant;        by inclusion of a polar solvent component d) to form a depot        precursor formulation. Use of a polar solvent in such a method        forms a further aspect.

The pre-formulations and components of the mixture, as well as theirperformance etc will evidently correspond to those described herein forother aspects.

Similarly, the present invention provides a method for improving therelease profile of a peptide somatostatin receptor agonist comprisingpasireotide from a depot composition formed by injection of a lowviscosity mixture comprising:

-   -   a) 20-50 wt. % of at least one diacyl glycerol;    -   b) 20-54 wt. % of at least one phosphatidyl choline (PC);    -   c) 5-15 wt. % of at least one biocompatible, organic        mono-alcoholic solvent;    -   e) 5 to 150 mg/ml of at least one peptide somatostatin receptor        agonist comprising pasireotide;    -   f) optionally at least one antioxidant;        by inclusion of a polar solvent component d) in said        low-viscosity mixture to form a depot precursor formulation. Use        of a polar solvent in such a method forms a further aspect.

The pre-formulations and components of the mixture, as well as theirperformance etc will evidently correspond to those described herein forother aspects.

Corresponding methods and uses provide for the reduction ofinjection-site discomfort, reduction of viscosity of thepre-formulation, and/or reduction in initial “burst” release of a lowviscosity mixture comprising:

-   -   a) 20-50 wt. % of at least one diacyl glycerol;    -   b) 20-54 wt. % of at least one phosphatidyl choline (PC);    -   c) 5-15 wt. % of at least one biocompatible, organic        mono-alcoholic solvent;    -   e) 5 to 150 mg/ml of at least one peptide somatostatin receptor        agonist comprising pasireotide;    -   f) optionally at least one antioxidant;        by inclusion of a polar solvent component d) in said        low-viscosity mixture to form a depot precursor formulation. Use        of a polar solvent in such a method forms a further aspect.

All of the above uses and methods for improving the various propertiesof the pre-formulation and/or the resulting depot composition arepreferably applied without negatively affecting the release profile ofthe peptide somatostatin receptor agonist.

The peptide somatostatin receptor agonist comprises pasireotide, hencesuitable doses for inclusion in the formulation, and thus the volume offormulation used, will depend upon the release rate (as controlled, forexample by the solvent type and amount used, the antioxidant content andso forth) and release duration, as well as the desired therapeuticlevel, the activity of the specific agent, and the rate of clearance ofthe particular active chosen. Typically an amount of around 0.05 to 40mg per week of depot duration, preferably 0.1 to 20 mg per week duration(e.g. 1 to 5 mg per week) for a duration of 1 to 24 weeks, preferably 2to 16 (e.g. 3, 4, 8, 10 or 12) weeks. In an alternative embodiment thepre-formulation may be formulated for dosing weekly (e.g. every 7±1days). A total dose of 0.05 to 250 mg per dose would be suitable forproviding a therapeutic level for between 7 and 168 days. This willpreferably be 0.1 to 200 mg, e.g. 0.2 to 150 mg, 0.1 to 100 mg, 20 to160 mg etc. Evidently, the stability of the active and effects on therelease rate will mean that the loading to duration may not be a linearrelationship. A depot administered every 30 days might have, for example0.2 to 20 mg, or a 90 day depot might have 30 to 60 mg of somatostatinreceptor agonist.

Evidently also, the biological half-life of the specific active will beparticularly important. The half-life of somatostatin, is less than 5minutes, and so for sustained release, a relatively large amount (e.g.towards the higher end of the range) will be needed. For an analoguesuch as pasireotide, with a much longer half-life (2-3 hours at least),the amount needed will evidently be lower. Appropriate levels for thespecific actives will be established easily by those of skill in the artby reference to the known therapeutic level, the desired duration ofaction and the volume which is to be injected. A good base calculationwould be to multiply a typical daily dose of the active agent by thenumber of days' duration of the depot. The formulation can then betested for linearity of release and adjusted as appropriate.

In a highly preferred embodiment, the lipid matrix aspect is soy PC or“high purity” PC (such as DOPC), GDO, ethanol, and water/propyleneglycol or mixtures thereof, and the peptide somatostatin receptoragonist comprises pasireotide. As indicated above, appropriate amountsof each component suitable for the combination are those amountsindicated herein for the individual components, in any combination.

In one preferred embodiment, GLP-1, GLP-1 analogues and GLP-1 receptoragonists and/or GLP-1 receptor antagonists are not present in theprecursor formulations of the invention.

Optional Component f)—Antioxidant

Component f) is an antioxidant. Most preferably it is selected fromascorbic acid, ascorbyl palmitate, ethylenediaminetetraacetic acid(EDTA) and salts thereof and citric acid.

In all aspects of the invention, component f) is typically present at aweight ratio of antioxidant to peptide somatostatin receptor agonist of1:50 to 1:6000, preferably 1:100 to 1:1300, and most preferably 1:150 to1:1250. Since typical antioxidants are of lower molecular weight thatthe peptide somatostatin receptor agonists, the proportion by weight ofantioxidant may be relatively small. For example, with a small molecularweight antioxidant (e.g. less than 500 amu), 0.0001 to 0.5% of thecomposition may be antioxidant, preferably 0.0005 to 0.2%, morepreferably 0.0008 to 0.1%, e.g. 0.001 to 0.02%.

Unfortunately, many common antioxidants are not highly compatible withlipid systems. Indeed, the present inventors have previously establishedthat some antioxidants commonly used in previous systems can causeincreased degradation of active agents in a lipid system. This appliesparticularly to peptide active agents. The present inventors havetherefore analysed a variety of potential antioxidant compounds andclasses for use with lipid based matrix systems and have surprisinglyfound that one particular class of antioxidants is unusually well suitedfor use in these systems.

The antioxidant component is generally included in the range 0.0001 to0.5% by weight of the total pre-formulation. Around 0.0005 to 0.015% ofantioxidant (particularly EDTA) is particularly preferred, especially incombination with the other preferred components and ranges indicatedherein above and below.

Stability data using a number of different antioxidants demonstrate thatEDTA antioxidants are surprisingly more efficient than otherantioxidants in suppressing the oxidative degradation of bioactiveagents. EDTA as antioxidant can also show a synergistic effect incombination with the antioxidants of the present invention, inmaintaining the chemical and physical stability of the peptide activeagent and complete pre-formulation. EDTA has a stabilising effect on theactive agent.

By “stabilising” is indicated an increase in the physical and chemicalstability of the dissolved or dispersed somatostatin receptor agonist.An increase in stability may be demonstrated by the chemical and/orphysical stability of a peptide somatostatin receptor agonist in a lipidformulation for a greater period than would be observed in the absenceof an antioxidant. This would preferably be tested under conditions oftypical storage, such as 2-8° C., 25° C. and/or ambient temperature.This is further described herein below.

In a preferred embodiment of the invention, antioxidants are excludedfrom the pre-formulations.

Optional Additional Components

In one particularly preferred embodiment of the present invention, thecompositions (preformulations and resulting depots) do not includefragmentation agents, such as polyethyleneoxide or poly(ethylene glycol)(PEG) fragmentation agent, e.g. a PEG grafted lipid and/or surfactant.

For example, the compositions preferably do not include fragmentationagents such as Polysorbate 80 (P80, polyoxyethylene (20) sorbitanmonooleate), or other Polysorbates (e.g. Polysorbate 20), PEGylatedphospholipids (PEG-lipids such as DSPE-PEG(2000), DSPE-PEG(5000),DOPE-PEG(2000) and DOPE-PEG(5000)), Solutol HS 15, PEGylated fatty acids(e.g. PEG-oleate), block co-polymers such as Pluronic® F127 andPluronic® F68, ethoxylated castor oil derivatives (e.g. Chremophores),PEGylated glyceryl fatty acid esters (such as TMGO-15 from NikkoChemicals) and PEGylated tocopherols (such as d-alpha tocopherylpoly(ethylene glycol) 1000 succinate known as Vitamin E TPGS fromEastman.

Single-dose formats must remain stable and potent in storage prior touse, but are disposable after the single use. In one embodiment, asingle dose format is stable at refrigerated conditions (e.g. 0-5 or2-8° C.) for at least 12 months. Furthermore such a pre-formulation maybe stable at room temperature (e.g. 25° C.) for at least 12 months.Multi-dose formats must not only remain stable and potent in storageprior to use, but must also remain stable, potent and relatively free ofbacteria (and particularly essentially free of bacterial growth) overthe multiple-dose use regimen administration period after the first usein which a seal has been compromised. For this reason multi-dose formatsoften require an anti-microbial or microbial-static agent, e.g.bacteriostatic agent, preservative.

However, the production of preserved pharmaceutical preparationscontaining protein or peptide actives has often proven difficult, aswhen preservatives are used, these give rise to stability problems.Often the proteins are inactivated and aggregates are formed, which maysometimes lead to reported injection site intolerance or immunogenicityto the active. This can be further aggravated by additional excipientsor formulation components.

In one aspect each of the embodiments herein can optionally contain anantimicrobial or microbial-static agent, which includes bacteriostaticagents and preservative. Such agents include benzalkonium chloride,m-cresol, benzyl alcohol or other phenolic preservatives. Typicalconcentrations as known in the art can be used.

Additional components above those mentioned as components a) to f) will,where present at all, preferably be present in an amount of 0 to 5%(e.g. 0.01% to 5%) by weight, preferably no more than 2% by weight andmore preferably no more than 1% by weight.

In one embodiment, components a) and b) (allowing for any impurityinherent in the nature of these components) make up at least 95% of thelipid components of the composition. Preferably at least 99% of thetotal lipid content of the pre-formulation consists of components a) andb). Preferably the lipid component of the pre-formulation consistsessentially of components a) and b).

Administration

The pre-formulations of the present invention are generally formulatedto be administered parenterally. This administration will generally notbe an intra-vascular method but will preferably be subcutaneous (s.c.),intracavitary or intramuscular (i.m.). Typically the administration willbe by injection, which term is used herein to indicate any method inwhich the formulation is passed through the skin, such as by needle,catheter or needle-less (needle-free) injector. Preferred parenteraladministration is by i.m or s.c. injection, most preferably by s.c.injection. An important feature of the composition of the invention isthat it can be administered both by i.m. and s.c. and other routeswithout toxicity or significant local effects. It is also suitable forintracavital administration. The s.c. injection has the advantage ofbeing less deep and less painful to the subject than the (deep) i.m.injection used for some current depots and is technically most suitablein the present case as it combines ease of injection with low risk oflocal side effects. It is a surprising observation of the presentinventors that the formulations provide sustained release of activeagent over a predictable time period by both subcutaneous andintramuscular injection. This therefore allows the site of injection tobe varied widely and allows the dose to be administered without detailedconsideration of the tissue depth at the site of injection.

The preferred lipid pre-formulations of the present invention providenon-lamellar liquid crystalline depot compositions upon exposure toaqueous fluids, especially in vivo. As used herein, the term“non-lamellar” is used to indicate a normal or more preferably reversedliquid crystalline phase (such as a reversed cubic or hexagonal phase)or the L3 phase or any combination thereof. The term liquid crystallineindicates all hexagonal, all cubic liquid crystalline phases and/or allmixtures thereof. Hexagonal as used herein indicates “normal” or“reversed” hexagonal (preferably reversed) and “cubic” indicates anycubic liquid crystalline phase unless specified otherwise. The skilledreader will have no difficulty in identifying those compositions havingappropriate phase behaviour by reference to the description and Examplesprovided herein, and to WO2005/117830, but the most favouredcompositional area for phase behaviour is where ratio of components a:bare in the region of 40:60 to 70:30, preferably 45:55 to 55:45 and morepreferably 40:60 to 54:46. Ratios of around 50:50 (e.g. ±2) are highlypreferred for most formulations (although certain exceptions are notedherein), most preferably around 50:50.

It is important to appreciate that the pre-formulations of the presentinvention are of low viscosity. As a result, these pre-formulations mustnot be in any bulk liquid crystalline phase since all liquid crystallinephases have a viscosity significantly higher than could be administeredby syringe or similar injecting dispenser. The pre-formulations of thepresent invention will thus be in a non-liquid crystalline state, suchas a solution, L₂ or L₃ phase, particularly solution or L₂. The L₂ phaseas used herein throughout is preferably a “swollen” L₂ phase containinggreater than 5 wt %, preferably greater than 7%, and most preferablygreater than 9% of organic mono-alcoholic solvent (component c) having aviscosity reducing effect. The pre-formulations of the invention whichare in L₂ phase form one preferred set of pre-formulations and thesewill generally contain at least 2% water as polar solvent.

As used herein, the term “low viscosity mixture” is used to indicate amixture which may be readily administered to a subject and in particularreadily administered by means of a standard syringe and needlearrangement. This may be indicated, for example by the ability to bedispensed from a 1 ml disposable syringe through a small gauge needle.Preferably, the low viscosity mixtures can be dispensed through a needleof 19 gauge, preferably smaller than 19 gauge, more preferably 23 gauge(or most preferably even 27 gauge) needle by manual pressure. In aparticularly preferred embodiment, the low viscosity mixture should be amixture capable of passing through a standard sterile filtrationmembrane such as a 0.22 μm syringe filter. A typical range of suitableviscosities would be, for example, 0.1 to 5000 mPas, preferably 1 to1000 mPas, more preferably 10 to 750 mPas and most preferably 25 to 500mPas at 20° C.

It has been observed that by the addition of small amounts of lowviscosity organic mono-alcoholic solvent, as indicated herein, a verysignificant change in viscosity can be provided. For example, theaddition of only 5% solvent to a lipid mixture can reduce viscosity100-fold and addition of 10% may reduce the viscosity up to 10,000 fold.In order to achieve this non-linear, synergistic effect in loweringviscosity it is important that a solvent of appropriately low viscosityand suitable polarity be employed. Such solvents include those describedherein infra. Preferred low-viscosity mixtures include molecularsolutions, including dispersions of the peptide somatostatin receptoragonist in a molecular solution of the other components.

Upon administration, the preferred lipid-based pre-formulations of thepresent invention undergo a phase structure transition from a lowviscosity mixture to a high viscosity (generally tissue adherent) depotcomposition. Generally this will be a transition from a molecularmixture, swollen L₂ and/or L₃ phase to one or more (high viscosity)liquid crystalline phases such as reversed hexagonal or cubic liquidcrystalline phases or mixtures thereof. Further phase transitions mayalso take place following administration. Obviously, complete phasetransition is not necessary for the functioning of the invention but atleast a surface layer of the administered mixture will form a liquidcrystalline structure. Generally this transition will be rapid for atleast the surface region of the administered formulation (that part indirect contact with air, body surfaces and/or body fluids). This willmost preferably be over a few seconds or minutes (e.g. from 1 second upto 30 minutes, preferably up to 10 minutes, more preferably 5 minutes ofless). The remainder of the composition may change phase to a liquidcrystalline phase more slowly by diffusion and/or as the surface regiondisperses.

Without being bound by theory, it is believed that upon exposure toexcess aqueous fluid, the pre-formulations of the invention lose some orall of the organic solvent included therein (e.g. by diffusion) and takein aqueous fluid from the bodily environment (e.g. the in vivoenvironment). For lipid pre-formulations, at least a part of theformulation preferably generates a non-lamellar, particularly liquidcrystalline phase structure. In most cases these non-lamellar structuresare highly viscous and are not easily dissolved or dispersed into the invivo environment. The result is a monolithic “depot” generated in vivowith only a limited area of exposure to body fluids. Furthermore,because the non-lamellar structure has large polar, apolar and boundaryregions, the lipid depot is highly effective in solubilising andstabilising active agents such as peptides and protecting these fromdegradation mechanisms. As the depot composition formed from thepre-formulation gradually degrades over a period of days, weeks ormonths, the active agent is gradually released and/or diffuses out fromthe composition. Since the environment within the depot composition isrelatively protected, the pre-formulations of the invention are highlysuitable for active agents with a relatively short biological half-life(see above).

By incorporation of at least 5% (e.g. at least 10%) of a polar solvent(especially at least 5% water and/or PG) into the pre-formulations, itis believed that the rate of phase transition to a non-lamellar (e.g.liquid crystalline) phase at the surface of the injected pre-formulationcan be enhanced in comparison with compositions containing organicsolvents in the substantial absence of water. The performance of theresulting depot is thus improved and further control over the release ofactive agent achieved.

The depot systems formed by the formulations of the present inventionare highly effective in protecting the active agent from degradation andthus allow an extended release period. The formulations of the inventionthus may provide in vivo depots of peptide somatostatin receptoragonists which require administration only once every 5 to 90 dayspreferably 5 to 60 days, more preferably 6 to 32. Evidently, a longerstable release period is desirable for patient comfort and compliance,as well as demanding less time from health professionals if thecomposition is not to be self-administered. Where the composition is tobe self-administered, patient compliance may be aided by a weekly (e.g.every 7 days, optionally ±1 day) or monthly (e.g. every 28 or 30 days(optionally ±7 days) administration so that the need to administer isnot forgotten.

A considerable advantage of the depot precursors of the presentinvention is that they are stable homogeneous phases. That is to say,they may be stored for considerable periods (preferably at least 6months) at room or refrigerator temperature, without phase separation.As well as providing advantageous storage and facile administration,this allows for the dose of peptide somatostatin receptor agonist (e.g.pasireotide) to be selected by reference to the species, age, sex,weight, and/or physical condition of the individual subject, by means ofinjecting a selected volume.

The present invention thus provides for methods comprising the selectionof a dosing amount specific to an individual, particularly by subjectweight. The means for this dose selection is the choice ofadministration volume.

In one preferred aspect, the present invention provides apre-formulation comprising components a), b), c), d), f) and at leastone peptide somatostatin receptor agonist comprising pasireotide asindicated herein. The amounts of these components will typically be inthe range 30-60% a), 30-70% b), 5-20% c) and 1-20% d), with the peptidesomatostatin receptor agonist comprising pasireotide present at 0.01% to10%, (such as 36-44% a), 36-44% b), 3-18% c) and 5-18% d) (preferablyincluding at least 2% water), with the peptide somatostatin receptoragonist comprising pasireotide present at 1% to 8%), wherein the ratioof a:b is in the range 40:60 to 54:46.

Typically, component f) is present at an antioxidant to peptidesomatostatin receptor agonist molar ratio of 1:50 to 1:6000, preferably1:100 to 1:1300, and most preferably 1:150 to 1:1250. Since typicalantioxidants are of lower molecular weight than peptide somatostatinreceptor agonist (e.g. somatostatin analogue, e.g. octreotide), theproportion by weight of antioxidant may be relatively small. Forexample, with a small molecular weight antioxidant (e.g. less than 500amu), 0.001 to 5% of the composition may be antioxidant, preferably0.002 to 2%, more preferably 0.002 to 0.15%, e.g. 0.002 to 0.015%.

The pre-formulations of the present invention are highly advantageous inthat they are stable to prolonged storage in their final “administrationready” form. As a result, they may readily be supplied foradministration either by health professionals or by patients or theircarers, who need not be fully trained health professionals and may nothave the experience or skills to make up complex preparations. This isparticularly important in long-duration, slow-effecting diseases such asdiabetes.

The pre-formulation of the invention will preferably exclude any GLP-1,GLP-1 analogues and GLP-1 receptor agonists and/or antagonists. Thepre-formulations of the invention will preferably exclude the followingpre-formulations:

GLP-1/ PC/ GDO3/ EtOH/ H₂O/ Formulation wt % wt % wt % wt % wt % Excl-K0.5 35.775 43.725 10 10 Excl-L 1.0 35.55 43.45 10 10 Excl-M 2.0 37.3545.65 5 10 Excl-N 2.0 32.85 40.15 10 15 Excl-O 2.0 30.4 45.6 10 12Excl-P 3.0 30 45 10 12 Excl-Q 3.0 31.875 43.125 10 12 Excl-R 3.0 32.439.6 10 15 Excl-T  2.0* 32.85 40.15 10 15 Excl-U  2.0* 30.4 45.6 10 12where EtOH is ethanol, PC is LIPOID S100 soybean phosphatidylcholine orLIPOID E 80 egg phosphatidylcholine (marked with *) and GDO is glyceroldioleate having quality (according to AC) as follows:

GDO quality (according to AC) Monoglycerides Diglycerides TriglyceridesGDO3 0.5% 95.3% 4.0%

Devices

In a yet further aspect, the present invention provides a disposableadministration device (which is also to include a device component)pre-loaded with a measured dose of a pre-formulation of the presentinvention. Such a device will typically contain a single dose ready foradministration, and will generally be sterile-packed such that thecomposition is stored within the device until administration. Suitabledevices include cartridges, ampoules and particularly syringes andsyringe barrels, either with integral needles or with standard (e.g.luer) fittings adapted to take a suitable disposable needle. Similarlyappropriate devices include a needle-less injector, a multi- orsingle-use autoinjector combined with a pre-filled syringe, a cartridge,optionally combined with a multi-use pen device, or a vial. Evidently,such pre-filled syringes and cartridges may be for any appropriateinjecting device, such as a multi-use or single-use injector orneedle-less injection unit.

The devices of the invention may preferably contain the pre-formulationof the invention which delivers a dosage in the range of 5 to 150 mg/ml,preferably 10 to 100 mg/ml, most preferably 10 to 70 or 10 to 90 mg/ml,for example 20 to 60 or 20 to 80 mg/ml, such as 20 to 60 or 30 to 60mg/ml.

In one embodiment applicable to all aspects of the invention, thedevices of the invention may contain a single dose of 1 to 200 mg, forexample 1 to 150 mg (e.g. 1 to 120 mg) of peptide somatostatin receptoragonist comprising pasireotide, preferably pasireotide pamoate.

The devices of the invention may contain peptide somatostatin receptoragonist comprising pasireotide, preferably pasireotide pamoate, ataround 0.1 to 6 mg (e.g. 0.2 to 4 mg) per day between scheduledadministrations, for example around 0.6 (e.g. 0.6 to 3) mg per day,particularly 1 to 2 mg/day.

The devices of the invention may contain a total volume foradministration of no more than 5 ml, for example no more than 2 ml, suchas approximately 1.5 ml.

The pre-filled devices of the invention may also suitably be included inan administration kit, which kit also forms a further aspect of theinvention. In a still further aspect, the invention thus provides a kitfor the administration of at least one peptide somatostatin receptoragonist comprising pasireotide, said kit containing a measured dose of aformulation of the invention and optionally an administration device orcomponent thereof. Preferably the dose will be held within the device orcomponent, which will be suitable for i.m. or preferably s.c.administration. The kits may include additional administrationcomponents such as needles, swabs, etc. and will optionally andpreferably contain instructions for administration. Such instructionswill typically relate to administration by a route as described hereinand/or for the treatment of a disease indicated herein above.

Kits

The invention provides for a pre-filled administration device asindicated herein and a kit as indicated herein comprising apre-formulation as described herein.

In an alternative aspect of the present invention, the “kit” may containat least two vessels, a first containing a low viscosity mixture ofcomponents a) to d), as described here, and a second containing ameasured dose of at least one peptide somatostatin receptor agonistcomprising pasireotide as described herein. The antioxidant component f)may be formulated with the active agent, or more preferably as part ofthe low viscosity mixture, which will then comprise components a) to d)and f).

Such a “two component kit” may comprise the peptide somatostatinreceptor agonist as a powder formulation in one vial or pre-filledsyringe and components a) to d) (and optionally f)) in a second vial orpre-filled syringe. In the case of two syringes, before injection, thepre-filled syringes are connected and the powder comprising active agentis mixed with the matrix formulation by moving the syringe barrels backand forth, forming a solution or suspension which is injected.Alternatively, the liquid lipid formulation is drawn from one vial, oris pre-filled into a syringe, and is injected into a vial containingpeptide powder. This formulation may subsequently be mixed by handshaking or other suitable reconstitution method (e.g. vortex mixingetc.). The solvent component may be present in either or both vessels(e.g. vials or syringes). Where the solvent is at least partiallyconstituted with the active agent, this will generally be in the form ofa solution or suspension.

In this aspect, the invention therefore provides a two component kitcomprising

-   -   i) a first vessel containing a low viscosity mixture of        components a) to d) as described herein;    -   ii) a second vessel containing at least one peptide somatostatin        receptor agonist comprising pasireotide,    -   iii) an antioxidant component f) optionally in a third vessel,        preferably in the second vessel, or most preferably in the first        vessel;    -   iv) optionally and preferably at least one of:        -   1) at least one syringe (which may be one or both of said            first and second vessels);        -   2) a needle for administration, such as those described            herein;        -   3) instructions for generation of a composition of the            invention from the contents of the first and second vessels;        -   4) instructions for administration, whereby to form a depot            as described herein.

Preferred Features and Combinations

In combination with the features and preferred features indicatedherein, the pre-formulations of the invention may have one or more ofthe following preferred features independently or in combination:

All proportions indicated herein may optionally be varied by up to 10%of the amount specified, optionally and preferably by up to 5%;

Component a) comprises, consists essentially of or preferably consistsof GDO;

Component b) comprises, consists essentially of or preferably consistsof soy PC and/or “high purity PC” such as DOPC;

Component c) comprises, consists essentially of or preferably consistsof a 1, 2, 3 or 4 carbon alcohol, preferably isopropanol or morepreferably ethanol;

Component d) comprises, consists essentially of or preferably consistsof a polar solvent such as water, propylene glycol, or mixtures thereof;

Component f) comprises, consists essentially of or preferably consistsof ascorbic acid, ascorbyl palmitate, ethylenediaminetetraacetic acid(EDTA), and/or citric acid;

The pre-formulation contains at least one peptide somatostatin receptoragonist comprising pasireotide, preferably pasireotide pamoate;

The pre-formulation has a low viscosity as indicated herein.

The pre-formulation comprises forms a liquid crystalline phase asindicated herein upon in vivo administration.

The pre-formulation generates a depot following in vivo administration,which depot releases at least one somatostatin receptor agonist at atherapeutic level over a period of at least 7 days, preferably at least28 days, more preferably at least 60 days.

The pre-formulation has a higher loading of peptide somatostatinreceptor agonist comprising pasireotide than is stable in the sameformulation in the absence of component d)

The pre-formulation has a higher loading of peptide somatostatinreceptor agonist comprising pasireotide than is obtainable byequilibration at 25° C. of the same formulation in the absence ofcomponent d).

In combination with the features and preferred features indicatedherein, the method(s) of treatment of the present invention may have oneor more of the following preferred features independently or incombination:

The method comprises the administration of at least one formulation withone or more preferred features as indicated above;

The method comprises the administration of at least one formulation asindicated herein by i.m., s.c. or preferably deep s.c. injection;

The method comprises administration by means of a pre-filledadministration device as indicated herein;

The method comprises administration through a needle no larger than 20gauge, preferably smaller than 20 gauge, and most preferably 23 gauge orsmaller;

The method comprises a single administration every 20 to 100 days,preferably 28 to 60 days (for example 30-45 days).

In combination with the features and preferred features indicatedherein, the use(s) of the pre-formulations indicated herein in themanufacture of medicaments may have one or more of the followingpreferred features independently or in combination:

The use comprises the use of at least one formulation with one or morepreferred features as indicated above;

The use comprises the manufacture of a medicament for administration ofat least one formulation as indicated herein by i.m., s.c. or preferablydeep s.c. injection;

The use comprises the manufacture of a medicament for administration bymeans of a pre-filled administration device as indicated herein;

The use comprises the manufacture of a medicament for administrationthrough a needle no larger than 20 gauge, preferably smaller than 20gauge, and most preferably 23 gauge or smaller;

The use comprises the manufacture of a medicament for administrationonce every 20 to 100 days, preferably 28 to 60 days, more preferably 30to 45 days.

In combination with the features and preferred features indicatedherein, the pre-filled devices of the invention may have one or more ofthe following preferred features independently or in combination:

They contain a preferred formulation as indicated herein;

They comprise a needle smaller than 20 gauge, preferably no larger than23 gauge;

They contain a single dose of 1 to 300 mg of peptide somatostatinreceptor agonist comprising pasireotide, preferably 1 to 200 mg, morepreferably 5-150 mg, for example 10-100 mg, most preferably 20-70 mg andespecially preferably 30-60 mg.

They contain a homogeneous mixture of a composition of the invention inready-to-inject form.

They contain a formulation of components a) to c) for combination with apeptide somatostatin receptor agonist comprising pasireotide whereby toform a pre-formulation of the invention.

They contain a peptide somatostatin receptor agonist comprisingpasireotide for combination with a formulation of components a) to d)and optionally f), whereby to form a pre-formulation of the invention.

They contain a total volume for administration of no more than 5 ml,preferably no more than 3 ml, for example no more than 2 ml, morepreferably no more than 1.5 ml.

In combination with the features and preferred features indicatedherein, the kits of the invention may have one or more of the followingpreferred features independently or in combination:

They contain a preferred formulation as indicated herein;

They contain a pre-filled device as indicated herein;

They contain a needle smaller than 20 gauge, preferably no larger than23 gauge;

They contain a single dose of 1 to 300 mg of peptide somatostatinreceptor agonist comprising pasireotide, preferably 1 to 200 mg, morepreferably 5-150 mg, for example 10-100 mg, most preferably 20-70 mg andespecially preferably 30-60 mg;

They contain a “two compartment kit” comprising at least two vesselscontaining a lipid formulation of the invention and a peptidesomatostatin receptor agonist comprising pasireotide powder,respectively.

They contain a total volume for administration of no more than 5 ml,preferably no more than 3 ml, for example no more than 2 ml, morepreferably no more than 1.5 ml.

They contain instructions for administration by a route and/or at afrequency as indicated herein;

They contain instructions for administration for use in a method oftreatment as described herein.

The Invention will now be further illustrated by reference to thefollowing non-limiting Examples and the attached Figures.

EXAMPLES Materials

-   -   SPC Soy phosphatidylcholine (Lipid S100)—Lipoid    -   GDO Glycerol dioleate (Rylo DG19 Pharma)—Danisco    -   DOPC Dioleoyl phosphatidylcholine—NOF    -   EtOH Ethanol—Solveco    -   PG Propylene glycol—Fischer    -   WFI Water for injection—Apoteket    -   EDTA Ethylenediaminetetraacetic acid, disodium salt—Sigma        Aldrich    -   SOM230(Pm) SOM230 pamoate (Pasireotide pamoate)—Novartis Pharma    -   SOM230(Ac) SOM230 acetate (Pasireotide acetate)—Novartis Pharma

Example 1: Solubility Screening

Placebo lipid mixture formulations as well as formulations of the singlerespective lipid alone were prepared in 1OR vials according to Table 1.The sample size was 6 g. Samples of SOM230 (pasireotide pamoate) of therespective formulation type with a drug load of 3 wt % (corrected forpeptide purity and content) were prepared in 2R vials, except forFormulation type 15 where only 1 wt % S230 was evaluated. The sampleswere allowed to equilibrate at ambient room temperature; samples withformulation types 1-8 on end-over-end rotation and samples withformulation types 9-12 on magnetic stirring. The flow chart in FIG. 1describes the sample preparation process. The screening covered thefollowing formulation variables:

-   -   Lipid weight ratio (SPC/GDO weight ratio)    -   Co-solvent nature and concentration    -   Single lipid formulations    -   Single solvent (only PG)

TABLE 1 Compositions of placebo lipid formulations (wt %) used forsolubility screening. Formulation type SPC GDO EtOH PG WFI Comment 147.5 47.5 5 — — SPC/GDO = 50/50 wt/wt 2 38 57 5 — — SPC/GDO = 40/60wt/wt 3 57 38 5 — — SPC/GDO = 60/40 wt/wt 4 45 45 10 — — 10 wt % EtOH 542.5 42.5 15 — — 15 wt % EtOH 6 45 45 5 5 — 5 wt % PG 7 42.5 42.5 5 10 —10 wt % PG 8 40 40 5 15 — 15 wt % PG 9 42.5 42.5 7.5 7.5 — 1/1 EtOH/PG;Magnetic stirring 10 40 40 10 10 — 1/1 EtOH/PG; Magnetic stirring 11 4040 10 — 10 1/1 EtOH/WFI; Magnetic stirring 12 35 35 15 — 15 1/1EtOH/WFI; Magnetic stirring 13 78 — 11 11 — Single lipid (SPC) 14 — 7811 11 — Single lipid (GDO) 15 — — — 100 — PG only

The samples were studied by visual inspection and the appearance wasnoted. Additional SOM230 drug powder in steps of 1 wt % (corrected forpeptide purity and content according to CoA) was added to any homogenousand transparent sample with the exception of Formulation type 15. Mixingat ambient room temperature and visual observation continued afteraddition of more SOM230 drug powder.

The results of the solubility screening are summarized in Table 2. It isconcluded that a dose strength of up to at least 10 wt % orapproximately 100 mg SOM230 free base/mL is attainable for someformulation types. Furthermore, PG appears to be a relatively goodsolvent for SOM230 (>1 wt %) in comparison to the poor solubility inEtOH (<<1 wt %).

Higher drug loads of SOM230, up to 10 wt % (or approximately 100 mg/mL(corrected)), were achieved using a co-solvent combination of EtOH/PG atconcentrations of 10 wt % each. Formulations comprising EtOH only asco-solvent were not as effective as the combination of EtOH/PG orEtOH/WFI for SOM230 loading. Formulations comprising only the singlerespective lipid excipient (Formulation types 13 and 14) displayed lowerdrug loading capability (<3 wt %) compared to lipid mixtures withequivalent solvent composition indicating strong synergistic solubilityenhancing effects by combining the components of the formulation of theinvention.

TABLE 2 Results of the solubility screening. For formulationcompositions, see Table 1. Maximum SOM230 drug load (wt %) MaximumHighest concentration observed Formulation concentration withtransparent and homogenous type evaluated (wt %) sample (wt %)/Comment 13 Some non-dissolved material in the bottom of the vial remained 2 3Small amount of non-dissolved material remained 3 3 Small amount ofnon-dissolved material remained 4 4 3 5 5 4 (a few non-dissolvedparticles/ crystals remained at 5 wt %) 6 5 4 7 4 3 (one singleremaining particle at 4 wt %) 8 5 5 9 6 6 10 10 10 11 10 6 (somenon-dissolved material remained at 10 wt %) 12 4 3 (opalescent sample at4 wt %) 13 3 Turbid/opalascent and non-dissolved material remained at 3wt % 14 3 Turbid/opalascent, oil-like drops and non-dissolved materialremained at 3 wt % 15 1 1

The solubility screening results are summarized as follows:

-   -   Multiple formulation types were found to allow for drug load        levels of 30-60 mg/mL    -   Increased co-solvent levels and combination of EtOH and PG or        EtOH and water increased SOM230 solubility    -   A drug load of (at least) 10 wt % (ca 100 mg/mL) was verified        for at least one formulation variant    -   The combination of SPC and GDO increased SOM230 solubility in a        synergistic way compared with the single lipid mixtures    -   PG appeared to be a relatively good solvent for SOM230

Example 2—Injectability, Density and Viscosity

Formulations according to Table 3 were prepared and used for evaluationof injectability, density and viscosity. The lipid/SOM230 formulationswith drug loads of 3 and 6 wt %, respectively, were prepared in 15Rinjection glass vials.

TABLE 3 Compositions of lipid/SOM230 formulations (wt %). Sample IDSOM230* SPC DOPC GDO EtOH PG WFI 4071S230- 4.31 40.3 — 40.4 7.5 7.5 —1201-60 4071S230- 4.31 37.8 — 37.8 10.0 10.0 — 1201-61 4071S230- 4.3137.8 — 37.8 10.1 — 10.0 1201-62 4071S230- 8.54 38.2 — 38.2 7.5 7.5 —1201-63 4071S230- 8.61 35.7 — 35.7 10.0 10.0 — 1201-64 4071S230- 8.6435.6 — 35.7 10.0 — 10.0 1201-65 4071S230- 8.60 34.7 — 34.7 12.0 — 10.01204-82 4071S230- 8.60 33.2 — 33.2 15.0 — 10.1 1204-83 B4071S230- 5.9139.55 — 39.55 7.50 7.50 — 1206-09 B4071S230- 7.39 38.81 — 38.81 7.507.50 — 1206-10 B4071S230- 5.91 — 39.55 39.55 7.50 7.50 — 1206-11B4071S230- 5.91 39.55 — 39.55 10.00 5.00 — 1206-12 *Pamoate salt; 4.3,5.9, 7.4 and 8.6 wt % SOM230 pamoate salt corresponds to ca 30, 40, 50and 60 mg SOM230 free base/g, respectively, when corrected for peptidepurity and content.

The preparation procedure of the samples was as described in FIG. 1 . Amagnetic stirring bar was added to the samples (sample size 6 g)followed by magnetic stirring at ambient room temperature. The sampleswere studied by visual inspection and the approximate time to completedissolution was noted.

The formulations with 30 mg/g were transparent and homogenous within 48h. The samples with 60 mg/g were homogenous and transparent within 3days. No special efforts (such as increasing the stirring rate) weremade to speed up dissolution times for these preparations.

Injectability (Flow Rates)

The injectability or flow rate of each formulation was evaluatedaccording to the following method: a constant force is applied to theselected syringe configuration filled with the respective formulation.The injection is then performed at ambient room temperature into anempty vial and the weight of the injected formulation is noted and thetime to complete the injection is measured. The injection volume perinjection test was approx. 0.4-0.6 mL and duplicate tests wereperformed. The constant force applied was 20 N. The syringe and needleformat used for the injectability tests are given in Table 4.

TABLE 4 Syringe and needle configuration. Syringe Supplied by NeedleSupplied by 1 mL long glass Gerresheimer 23G thin-wall Terumo Luer-Lock(tw), 16 mm

Stoppers used were from West (4432/50 grey B240 Westar®) with plungerrod 55103 supplied by Fresenius Kabi (FKA).

Injectability results are summarized in FIG. 2 . The injectability isgiven as seconds per mL (inverse of the flow rate). The values wereconverted from seconds per gram to seconds per mL by the use offormulation density values (see below). The time for injection, usingthe 20 N constant force, varied between approximately 8-30 secondsdepending on formulation.

Density

The density of each formulation was determined using an Anton Paardensity meter DMA 4500 M (1360) at 20° C. Single tests were performed oneach formulation. The results are presented in Table 5.

TABLE 5 Density measurements on lipid/SOM230 formulations. Formulationcompositions are provided in Table 3. Sample ID Density (g/mL)4071S230-1201-60 0.967 4071S230-1201-61 0.963 4071S230-1201-62 0.9644071S230-1201-63 0.979 4071S230-1201-64 0.975 4071S230-1201-65 0.9764071S230-1204-82 0.973 4071S230-1204-83 0.967 B4071S230-1206-09 0.971B4071S230-1206-10 0.975 B4071S230-1206-11 0.969 B4071S230-1206-12 0.965

Viscosity

The viscosity was measured using a Bohlin Visco 88 BV instrument(rotating inner cylinder, stationary outer cylinder) at three speedsettings (three shear rates). Single tests were performed for eachformulation.

The measurements were performed at room temperature (ambient). Theresults are shown in Table 6 as mean viscosity for the different speedsettings. No difference in viscosity values (within experimentalvariation) could be observed between the different shear ratesindicating Newtonian behaviour.

TABLE 6 Viscosity measurements on lipid/SOM230 formulations. Formulationcompositions are provided in Table 3. Sample ID Viscosity (mPas)4071S230-1201-60 308 4071S230-1201-61 168 4071S230-1201-62 3644071S230-1201-63 599 4071S230-1201-64 307 4071S230-1201-65 4254071S230-1204-82 284 4071S230-1204-83 142

It may be noted that by doubling the SOM230 drug load from ca 30 to 60mg/mL, the viscosity almost doubled for formulations with EtOH/PGwhereas the viscosity increase for the corresponding EtOH/WFIformulations was only about 20%. The link between injectability andviscosity is illustrated in FIG. 3 . As expected for these types offormulations, the injectability (calculated as time for 1 mL injection)is close to linearly proportional to viscosity.

Example 3—Comparative Formulation Studies with SOM230 Acetate and SOM230Hydrochloride Preparation of SOM230 Hydrochloride

The SOM230 hydrochloride salt was prepared from SOM230 acetate using anion exchange process. The ion exchange column was prepared by puttingglass wool (HPLC grade) in the bottom of a 200 mL glass chromatographycolumn from Sigma-Aldrich. A mixture of approximately 20 mL of Dowex 1×2chloride form (Sigma-Aldrich) and distilled water (volume ratio 1:1) wasadded followed by a piece of glass wool on the top of the column. Thecolumn was rinsed with distilled water and the conductivity wasmeasured. When the conductivity was below 35 μS/cm, a volume of 40 mLWFI was added to the column.

For the ion exchange, a SOM230 acetate (SOM230(Ac)) sample was preparedin a 100 mL Pyrex flask. The sample was diluted with WFI to a finalvolume of 65.55 mL and a concentration of 3.8 mg SOM230(Ac)/mL. TheSOM230(Ac) solution was transferred to the ion exchange column using aplastic disposable pipette. The Pyrex flask was rinsed with anadditional 20 mL of WFI which was also transferred to the column. Samplefractions of 20 mL were collected as the column was rinsed with WFI. Theconductivity was measured in each fraction and fractions were collecteduntil the conductivity was below 75 μS/cm. All fractions were pooledtogether in a 1000 mL round bottom flask (pooled volume ca 180 mL).

The round bottom flask from the previous step was kept at 2-8° C. untilfurther use. The flask was mounted on a Rotavapor and put onapproximately 80% of maximum rotation speed. The solution wasshell-frozen by lowering the rotating flask in an EtOH bath containingdry ice and a mixture of 99.5% and 96% EtOH (volume ratio 1:1) for 10minutes. After shell-freezing, the round bottom flask was kept at −80°C. for 30 min before the freeze-drying was started.

The material was freeze-dried for almost 30 h. The SOM230 hydrochloride(SOM230(Cl)) powder obtained was thereafter transferred and weighed intoa 250 mL Pyrex flask. The total amount of SOM230(Cl) recovered was 0.215g, resulting in a 86% yield of the ion exchange process. The peptidepowder was stored in a freezer (<−15° C.) until further use.

Pharmaceutical Analysis of SOM230 Hydrochloride

A comparison of the purity data (HPLC) for the SOM230(Ac) and theSOM230(Cl) indicated that the integrity of the SOM230 material remainedintact through the ion exchange process. The chloride content wasdetermined by HPLC to 5.15 wt % which corresponded well to thedetermined acetate content of 8.90 wt % in the original SOM230(Ac) drugpowder when the acetate/chloride weight ratio is taken into account. Theanalysis of the SOM230(Cl) drug powder did not detect any presence ofacetate ions, demonstrating a successful and complete ion exchangeprocess.

Solubility

Lipid formulations with SOM230(Ac) and SOM230(Cl), respectively, wereprepared as described in Example 1 according to Tables 8 and 9. Thetarget concentration was a SOM230 (free base) concentration ofapproximately 30 mg/mL.

TABLE 8 Compositions (wt %) of lipid/SOM230(Ac) formulations. Sample IDSOM230(Ac) SPC GDO EtOH PG WFI 4071S230(Ac)- 3.75 43.1 43.1 10.0 — —1203-120 4071S230(Ac)- 3.79 40.6 40.6 7.5 7.5 — 1203-121 4071S230(Ac)-3.75 38.0 38.0 10.0 10.0 — 1203-122 4071S230(Ac)- 3.77 38.1 38.1 10.0 —10.0 1203-123

TABLE 9 Compositions (wt %) of lipid/SOM230(Cl) formulations. Sample IDSOM230(Cl) SPC GDO EtOH PG WFI 4071S230(Cl)- 3.82 43.1 43.1 10.0 — —1203-124 4071S230(Cl)- 3.69 40.6 40.6 7.5 7.5 — 1203-125 4071S230(Cl)-3.72 38.0 38.0 10.0 10.0 — 1203-126 4071S230(Cl)- 3.84 38.1 38.1 10.0 —10.0 1203-127

The samples were allowed to equilibrate at room temperature on amagnetic stirrer (500 rpm) after brief vortex mixing. Visual inspectionwas performed after 1.5 and 24 hours. All formulations indicated inTables 8 and 9 were transparent and homogenous after 24 h mixingindicating good solubility of both the acetate and the hydrochloridesalt forms. Formulations of SOM230 pamoate (SOM230(Pm)) with identicallipid and co-solvent composition to those described in Tables 8 and 9were prepared according to the same procedure.

Stability Comparison

The lipid/SOM230(Pm), lipid/SOM230(Cl) and lipid/SOM230(Ac) formulationswere divided into two 2R vials which were placed at 60° C. and theremaining amount from the preparation was assayed by HPLC (time pointzero).

The samples stored at 60° C. were pulled out after 2 weeks of storagefor visual inspection and HPLC analysis.

The results of the HPLC analysis are provided in FIG. 4 and shows thedifference in stability profile between the salt forms for theinvestigated formulation variants (because the SPC/GDO weight ratio wasequivalent for all salt forms, formulation variants are differentiatedby their solvent composition in FIG. 4 ). The results after storage for2 weeks at 60° C. clearly indicate that SOM230 pamoate is the moststable salt form in the lipid formulations. The hydrochloride salt ofSOM230 was also more stable than the acetate salt but not as stable asthe pamoate salt.

Example 4—In Vivo PK Studies I and II Formulations

Formulations used for in vivo pharmacokinetic (PK) study I in rat (studyno. PK-12-437) are outlined in Table 10. A constant SOM230 load (pamoatesalt) corresponding to 30 mg SOM230 free base/mL was selected for allformulations. Combinations of different solvents, EtOH, EtOH/PG andEtOH/WFI, were investigated. The primary aim was to characterize the PKprofiles of the different formulation variants of SOM230 pamoate.

TABLE 10 Lipid/SOM230 formulations selected for PK study I (PK-12-437).Compositions are provided in wt %. SOM230 (pam- Batch no. Test itemoate)* GDO SPC EtOH PG WFI B4071S230- 4071S230- 4.45 40.28 40.28 7.507.50 — 1202-01 A B4071S230- 4071S230- 4.47 37.77 37.77 10.00 10.00 —1202-02 B B4071S230- 4071S230- 4.46 37.77 37.77 10.00 — 10.00 1202-03 CB4071S230- 4071S230- 4.48 42.76 42.76 10.00 — — 1202-04 D *The SOM230pamoate concentration corresponds to 30 mg SOM230 free base/mL whencorrected for peptide purity and content and formulation density.

Formulations used for in vivo PK study II in rat (study no. PK-12-438)are outlined in Table 11. The primary aim was to characterize effects ofincreasing the SOM230 load on the PK profile. For this study acombination of EtOH and water (WFI) was used as solvent for theformulations at a fixed concentration of 10 wt % of each component asindicated in Table 11. Formulation 4071S230-C was investigated in bothPK studies providing a bridge between the studies.

TABLE 11 Lipid/SOM230 formulations selected for PK study II (PK-12-438).Compositions are provided in wt %. S230 Batch no. Test item (pamoate)*GDO SPC EtOH WFI B4071S230- 4071S230- 4.46 37.77 37.77 10.00 10.001202-05 C B4071S230- 4071S230- 5.91 37.05 37.05 10.00 10.00 1202-06 EB4071S230- 4071S230- 7.39 36.31 36.31 10.00 10.00 1202-07 F B4071S230-4071S230- 8.81 35.60 35.60 10.00 10.00 1202-08 G *The SOM230 pamoateconcentrations correspond to 30, 40, 50 and 60 mg SOM230 free base/mL,respectively, when corrected for peptide purity and content andformulation density.

Manufacturing of the formulations in Tables 10 and 11 was performedessentially as described in Example 1 with the addition of a sterilefiltration step after complete mixing into homogenous liquidformulations. The formulations were sterile filtered under 2.5 barnitrogen pressure using a PVDF 0.2 micron membrane filter fromMillipore.

In Vivo Study Performance

The formulations in Table 10 (PK-12-437) were injected subcutaneously tomale Sprague-Dawley rats (body weight ca 330 g) at a dose volume of 0.2mL per animal (6 mg SOM230/animal) whereas the formulations in Table 11(PK-12-438) were injected at a dose volume of 0.1 mL/animalcorresponding to 3, 4, 5 and 6 mg SOM230/animal for 4071S230-C,4071S230-E, 4071S230-F and 4071S230-G, respectively. Blood forpharmacokinetics were collected pre-dose, and 1 hour, 6 hours, 1 day, 7days, 14 days, 21 days, 28 days and 35 days after dosing. Blood samplesof 0.5 mL were collected by sub-lingual bleeding into EDTA-treated testtubes (Capiject 3T-MQK, Terumo Medical Corporation). The blood wasplaced on ice immediately after collection and centrifuged(approximately 1500×g, at 5° C. for 10 min) within 30 to 60 minutes. Theplasma was transferred into properly labelled translucent 1.5-mLpropylene test tubes (Microcentrifuge tubes, Plastibrand, Buch & Holm)and stored below −70° C. until bioanalysis by ELISA assay.

Pharmacokinetics

The PK profiles of the respective formulations in Tables 10 and 11 areprovided in FIGS. 5 and 6 .

As is clear from the data in FIG. 5 , the PK profiles are very flat withCmax/C28d plasma concentration ratios between about 3.1-4.9, where Cmaxis the maximum plasma concentration observed and C28d is the plasmaconcentration observed at 28 days post injection. In terms ofCmax/Caverage ratios, where Caverage is the average plasma concentrationover the target 28 days duration, this ratio is even lower than therespective Cmax/C28d ratio. Thus, the initial release (or burst) is lowfollowed by consistent plasma levels, fulfilling the PK requirements foreffective depot formulations. The main PK-parameters obtained inPK-12-437 are tabulated in Table 12.

TABLE 12 PK parameters obtained in PK study no. PK-12-437. Formulationcompositions are provided in Table 10. AUClast Test item Dose (mg) Cmax(ng/mL) C28d (ng/mL) Cmax/C28d (ng/mL*d) 4071S230-A 6.0 187.7 ± 54.265.3 ± 34.2 3.4 3364 ± 806 4071S230-B 6.0 288.2 ± 98.2 70.2 ± 28.4 4.94550 ± 891 4071S230-C 6.0 190.2 ± 49.5 63.3 ± 13.1 3.1 3421 ± 8524071S230-D 6.0 150.7 ± 28.1  53 ± 24.1 3.4 2662 ± 864

The data in FIG. 6 show again that the release rate is consistent overtime resulting in very flat PK profiles. Cmax/C28d plasma concentrationratios were between about 3.4-9.2 for the formulations indicated inTable 11. In terms of Cmax/Caverage ratios, this ratio is even lowerthan the respective Cmax/C28d ratio. The main PK-parameters obtained inPK-12-438 are tabulated in Table 13.

TABLE 13 PK parameters obtained in PK study no. PK-12-438. Formulationcompositions are provided in Table 11. AUClast Test item Dose (mg) Cmax(ng/mL) C28d (ng/mL) Cmax/C28d (ng/mL*d) 4071S230-C 3.0 104.8 ± 39.829.2 ± 6.6 3.8 1893 ± 568 4071S230-E 4.0 185.3 ± 80.9 56.0 ± 7.4 3.42707 ± 918 4071S230-F 5.0  311 ± 88.9 103.9 ± 59.1 4.1 4649 ± 5054071S230-G 6.0  481.2 ± 100.3  53.2 ± 14.8 9.2  5742 ± 1021

Dose linearity with respect to exposure (AUC) was clearly indicated asshown in FIG. 7 (R²=0.977). Dose linearity with respect to Cmax was alsoshown as indicated in FIG. 8 (R2=0.975).

Example 5—Explorative Stability Testing

The formulations evaluated in the PK studies (Tables 10 and 11) werealso subjected to explorative stability testing. In addition to theseformulations, one additional formulation was manufactured comprising theantioxidant disodium ethylenediamine tetraacetic acid (EDTA). Theformulation composition of the additional formulation is provided inTable 14 (see Tables 10 and 11 for compositions of the otherformulations).

TABLE 14 Lipid/SOM230 formulation comprising antioxidant included in theexplorative stability testing. Composition in wt %. Batch/Sample SOM230no. (pamoate)* GDO SPC EtOH WFI/EDTA** 4071S230-1202- 4.46* 37.77 37.7710.00 10.00 109 *The SOM230 pamoate concentration corresponds to 30 mgSOM230 free base/mL when corrected for peptide purity and content andformulation density. **0.1 mg disodium EDTA/mL in WFI.

Summary of Explorative Stability Study Set-Up

Each formulation was filled in 2R vials with 0.8 g per vial followed byflushing with nitrogen for 5 seconds and closing with Teflon-coatedrubber stoppers and aluminum crimp caps. The storage conditions forformulations provided in Tables 10 and 14 were 5° C., 25° C./60% RH, 40°C./75% RH and 60° C. whereas the formulations provided in Table 11 wereonly evaluated at 5° C. and 25° C./60% RH. The samples were alwaysallowed to equilibrate for 60 min at ambient RT before start of the HPLCUV-DAD (diode array detection) analysis.

Results

The assayed (HPLC UV-DAD) peptide contents after up to 8 weeks ofstorage are summarized in Tables 15 and 16 whereas the peptide purityresults, calculated as the area of the SOM230 peak divided by the totalarea of the SOM230 peak and related substances, are summarized in Tables17 and 18.

TABLE 15 SOM230 content analysis (by HPLC UV-DAD) of formulations (seeTables 10 and 14) stored at 5, 25, 40 and 60° C. up to 8 weeks. t = 0 t= 2 weeks t = 4 weeks t = 8 weeks SOM230 SOM230 % of SOM230 % of SOM230% of Batch/ content Storage content start content start content startSample no. (mg/g) conditions (mg/g) value (mg/g) value (mg/g) valueB4071S230- 30.3  5° C. — — 31.9 105.3 31.4 103.7 1202-01 25° C./60% RH —— 31.8 105.0 30.8 101.7 40° C./75% RH 30.7 101.3  30.7 101.2 29.2  96.260° C. 27.1 89.5 25.1 82.9 — — B4071S230- 30.6  5° C. — — 32.5 106.331.3 102.4 1202-02 25° C./60% RH — — 32.1 104.9 31.0 101.3 40° C./75% RH30.9 100.8  31.6 103.2 29.2  95.4 60° C. 26.4 86.1 26.0 84.9 — —B4071S230- 30.7  5° C. — — 32.1 104.6 31.2 101.6 1202-03 25° C./60% RH —— 31.8 103.5 30.6  99.7 40° C./75% RH 30.4 99.0 29.2 101.6 29.3  95.360° C. 26.9 87.5 27.1 88.3 — — B4071S230- 28.7  5° C. — — 30.1 104.729.4 102.6 1202-04 25° C./60% RH — — 29.8 104.0 29.2 101.9 40° C./75% RH29.1 101.4  29.2 101.6 27.9  97.1 60° C. 25.9 90.3 23.4 81.6 — —4071S230- 30.4  5° C. — — 32.8 107.7 31.3 102.9 1202-109 25° C./60% RH —— 31.8 104.5 30.9 101.5 40° C./75% RH 30.7 100.9  31.2 102.7 30.0  98.660° C. 28.3 91.2 27.4 90.1 — —

TABLE 16 SOM230 content analysis (by HPLC UV-DAD) of formulations (seeTable 11) stored at 5 and 25° C. up to 8 weeks. t = 0 t = 4 weeks t = 8weeks SOM230 SOM230 % of SOM230 % of Batch/ content Storage contentstart content start Sample no. (mg/g) conditions (mg/g) value (mg/g)value B4071S230- 30.5 5° C. 31.1 106.0 31.0 101.8 1202-05 25° C./60% RH31.7 104.1 30.3 99.6 B4071S230- 40.9 5° C. 43.8 107.1 31.0 101.8 1202-0625° C./60% RH 42.8 104.6 30.3 99.6 B4071S230- 50.8 5° C. 53.7 105.5 51.9102.0 1202-07 25° C./60% RH 53.8 105.8 51.2 100.7 B4071S230- 59.9 5° C.64.2 107.2 60.7 101.3 1202-08 25° C./60% RH 63.3 105.6 61.5 102.6

TABLE 17 SOM230 purity analysis (by HPLC UV-DAD) of formulations (seeTables 10 and 14) stored at 5, 25, 40 and 60° C. up to 8 weeks. t = 0 t= 2 t = 4 t = 8 Rel. Area weeks weeks weeks Batch/ SOM230 Storage Rel.Area Rel. Area Rel. Area Sample no. (%) conditions SOM230 (%) SOM230 (%)SOM230 (%) B4071S230- 98.9 5° C. — 98.8 98.8 1202-01 25° C./60% RH —98.4 98.3 40° C./75% RH 98.1 96.9 96.1 60° C. 91.4 86.2 — B4071S230-98.9 5° C. — 98.7 98.8 1202-02 25° C./60% RH — 98.3 98.2 40° C./75% RH97.9 96.8 95.4 60° C. 90.1 85.4 — B4071S230- 99.1 5° C. — 98.8 98.91202-03 25° C./60% RH — 98.3 97.7 40° C./75% RH 97.0 95.6 94.9 60° C.90.4 85.8 — B4071S230- 98.6 5° C. — 98.7 98.7 1202-04 25° C./60% RH —98.3 98.1 40° C./75% RH 98.1 96.9 96.0 60° C. 91.3 85.7 — 4071S230- 99.15° C. — 99.0 99.1 1202-109 25° C./60% RH — 98.6 98.7 40° C./75% RH 98.597.6 96.3 60° C. 92.9 88.3 —

TABLE 18 SOM230 purity analysis (by HPLC UV-DAD) of formulations (seeTable 11) stored at 5 and 25° C. up to 8 weeks. t = 4 t = 8 t = 0 weeksweeks Batch/ Rel. Area Rel. Area Rel. Area Sample SOM230 Storage SOM230SOM230 no. (%) conditions (%) (%) B4071S230- 99.0 5° C. 98.8 99.01202-05 25° C./60% RH 98.1 97.8 B4071S230- 99.0 5° C. 98.8 99.0 1202-0625° C./60% RH 98.4 98.2 B4071S230- 99.1 5° C. 99.1 99.2 1202-07 25°C./60% RH 98.5 98.4 B4071S230- 99.1 5° C. 99.0 99.2 1202-08 25° C./60%RH 98.6 98.6

The following conclusions were drawn based on the peptide content andpurity data: No change in SOM230 content or purity at 5° C. was detected(within experimental variability).

Only small changes in the peptide content and purity were detected at25° C. Depending on formulation type, peptide purity decreased by2.6-4.2% after 8 weeks at 40° C. with a trend towards decreasingdegradation rate with storage time.

A positive effect of the inclusion of EDTA was detected as shown bycomparing B4071S230-1202-03 (without EDTA) and 4071S230-1202-109 (withEDTA).

Example 6—In Vivo PK study III (PK-12-451) Formulations

Formulations used for in vivo pharmacokinetic (PK) study III in rat(study no. PK-12-451) are outlined in Table 19. The SOM230concentrations investigated correspond to 40 mg and 50 mg SOM230 freebase/mL for the respective formulations. A combination of ethanol (EtOH)and propylene glycol (PG) was used for all formulations. The primary aimwas to characterize the PK profiles of the different formulationvariants of SOM230 pamoate.

TABLE 19 Lipid/SOM230 formulations selected for PK study III(PK-12-451). Compositions are provided in wt %. SOM230 Batch no. Testitem (pamoate)* SPC DOPC GDO EtOH PG B4071S230-1206-09 4071S230-H 5.9139.60 — 39.60 7.50 7.50 B4071S230-1206-10 4071S230-I 7.39 38.80 — 38.807.50 7.50 B4071S230-1206-11 4071S230-J 5.91 — 39.60 39.60 7.50 7.50B4071S230-1206-12 4071S230-K 5.91 39.60 — 39.60 10.00 5.00 *The SOM230pamoate concentration corresponds to 40 mg SOM230 free base/mL for4071S230-H, -J and -K and 50 mg/mL for 4071S230-I when corrected forpeptide purity and content and formulation density.

Manufacturing of the formulations in Table 19 was performed essentiallyas described in Example 1 with the addition of a sterile filtration stepafter complete mixing into homogenous liquid formulations. Theformulations were sterile filtered under 2.5 bar nitrogen pressure usinga PVDF 0.2 micron membrane filter from Millipore.

In Vivo Study Performance

The formulations in Table 19 (PK-12-451) were injected subcutaneously tomale Sprague-Dawley rats (body weight ca 330 g) at a dose volume of 0.1mL per animal (6 mg SOM230/animal) corresponding to 4 and 5 mgSOM230/animal for 4071S230-H, 4071S230-J, 4071S230-K and 4071S230-I,respectively. Blood for pharmacokinetic analysis was collected 1 hour, 6hours, 1 day, 3 days, 7 days, 14 days, 21 days, 28 days and 35 daysafter dosing. Blood samples of 0.5 mL were collected by sub-lingualbleeding into EDTA-treated test tubes (Capiject 3T-MQK, Terumo MedicalCorporation). The blood was placed on ice immediately after collectionand centrifuged (approximately 1500×g, at 5° C. for 10 min) within 30 to60 minutes. The plasma was transferred into properly labelledtranslucent 1.5-mL propylene test tubes (Microcentrifuge tubes,Plastibrand, Buch & Holm) and stored below −70° C. until bioanalysis byELISA assay.

The PK profiles of the respective formulations in Table 19 are providedin FIG. 10 .

As is clear from the data in FIG. 9 , the PK profiles are generally flatwith somewhat higher plasma levels over the first 14 days for4071S230-I. The Cmax/C28day plasma concentration ratios varied in therange 2.6-8.4 depending on formulation variant.

A noteworthy result is the fact that the lowest Cmax/C28day plasma levelratio and hence for this perspective the most attractive PK profile wasobtained for 4071S230-J comprising DOPC instead of SPC (see Table 19 forcompositions).

The main PK-parameters obtained in PK-12-451 are tabulated in Table 20.

TABLE 20 PK parameters obtained in PK study PK-12-451. Formulationcompositions are provided in Table 19. AUClast Test item Dose (mg) Cmax(ng/mL) C28d (ng/mL) Cmax/C28d (ng/mL*d) 4071S230-H 4.0 217.2 ± 52.940.5 ± 17.6 6.0 2550 ± 815 4071S230-I 5.0 352.5 ± 81.8 46.0 ± 9.6 8.44211 ± 742 4071S230-J 4.0 133.6 ± 53.0 47.7 ± 21.4 2.6 2274 ± 6304071S230-K 4.0 183.3 ± 55.1 40.7 ± 14.2 4.9 2347 ± 585

Example 7—Further Explorative Stability Testing Summary of ExplorativeStability Testing

The compositions of the formulations investigated in the stability studyare provided in Table 19. Each formulation was filled in 2R vials with1.0 g per vial followed by flushing with nitrogen for 5 seconds andclosing with Teflon-coated rubber stoppers and aluminium crimp caps. Thestorage conditions were 5° C. and 25° C./60% RH (ICH compliant). Thesamples were always allowed to equilibrate for 60 min at ambient RTbefore start of the HPLC UV-DAD (diode array detection) analysis.

SOM230 Purity Analysis (by HPLC UV-DAD) up to 12 Weeks

The results from the SOM230 purity analysis after storage up to 12 weeksare presented in FIGS. 10 and 11 . No change in the SOM230 purity wasdetected at 5° C. The total related substances (RS=100%−found peptidepurity) observed after 12 weeks at 25° C. was in the range of 1.4-1.9%with the starting values at release (time zero) being in the range from0.9-1.1%. The SOM230 (pamoate) drug powder was found to contain about0.7% related substances and hence this level should be taken as thereference level. Adjusted for the SOM230 drug powder reference level,the total related RS, or total degradation products, found after 12weeks at 25° C. was in the range of 0.7-1.3% whereas the increase oftotal RS up to 12 weeks, with time zero as reference, was in the range0.3-0.9% with the DOPC-based formulation (B4071S30-1206-11, see Table19) showing the lowest total RS.

Example 8—Further SOM230 Compositions Comprising DOPC

Lipid formulations of SOM230 comprising DOPC were prepared as describedin Example 1 resulting in homogenous liquids after the mixing process.The formulation compositions are provided in Table 21.

TABLE 21 Lipid/SOM230 formulations comprising DOPC. Compositions areprovided in wt %. SOM230 Sample no. (pamoate)* DOPC GDO EtOH PG4071S230-1210-204 8.64 38.13 38.14 7.48 7.61 4071S230-1210-205 8.6541.97 34.42 7.49 7.47 4071S230-1210-206 8.58 34.44 41.96 7.51 7.51 *TheSOM230 pamoate concentration corresponds to 60 mg SOM230 free base/mLwhen corrected for peptide purity and content and formulation density.

The formulations (0.2 g) were injected into 5 mL phosphate bufferedsaline (PBS pH 7.4) using a 1 mL disposable Luer-Lock syringe equippedwith a 23G thin wall 16 mm needle. All formulations formed coherentliquid crystal gels in contact with PBS.

Example 9—SOM230 Compositions Comprising DOPC and Different SolventContent

Lipid formulations of SOM230 comprising DOPC and different solventcontent were prepared essentially as described in Example 1 with theaddition of a sterile filtration step after complete mixing intohomogenous liquid formulations. The formulations were sterile filteredunder 2.5 bar nitrogen pressure using a PVDF 0.2 micron membrane filterfrom Millipore. The formulation compositions are provided in Table 22.

TABLE 22 Lipid/SOM230 formulations comprising DOPC and different solventcontent. Compositions are provided in wt %. Sample SOM230 SOM230 freeno. (pamoate) DOPC GDO EtOH PG base (mg/mL) B4071S30- 2.94 41.0 41.0 7.57.5 20 1302-13 B4071S30- 5.82 39.6 39.6 7.5 7.5 40 1302-14 B4071S30-8.65 38.2 38.2 7.5 7.5 60 1302-15 B4071S30- 8.65 42.0 34.4 7.5 7.5 601302-16 B4071S30- 8.65 39.2 32.1 10.0 10.0 60 1302-17 B4071S30- 8.6538.2 38.2 10.0 5.0 60 1302-18 9-1 2.94 41.0 41.0 10.0 5.0 20 9-2 5.8239.6 39.6 10.0 5.0 40

The formulations (0.2 g) were injected into 5 mL phosphate bufferedsaline (PBS pH 7.4) using a 1 mL disposable Luer-Lock syringe equippedwith a 23G thin wall 16 mm needle. All formulations formed coherentliquid crystal gels in contact with PBS.

Example 10—High Drug Load SOM230 Compositions

High loading lipid formulations of SOM230 comprising DOPC and differentsolvent content are prepared essentially as described in Example 1 withthe addition of a sterile filtration step after complete mixing intohomogenous liquid formulations.

The formulations are sterile filtered under 2.5 bar nitrogen pressureusing a PVDF 0.2 micron membrane filter from Millipore. The formulationcompositions are provided in Table 23.

TABLE 23 High SOM230 drug load lipid compositions Sample SOM230 SOM230free base no. (pamoate) DOPC GDO EtOH PG (mg/mL) 10-1 13.0 33.5 33.510.0 10.0 ca 90 10-2 13.0 36.9 30.1 10.0 10.0 ca 90 10-3 13.0 31.0 31.015.0 10.0 ca 90 10-4 13.0 34.1 27.9 15.0 10.0 ca 90 10-5 13.0 28.5 28.515.0 15.0 ca 90 10-6 13.0 31.4 25.6 15.0 15.0 ca 90

The formulations (0.2 g) are injected into 5 mL phosphate bufferedsaline (PBS pH 7.4) using a 1 mL disposable Luer-Lock syringe equippedwith a 23G thin wall 16 mm needle. All formulations form coherent liquidcrystal gels in contact with PBS.

1. A pre-formulation comprising a low viscosity mixture of: a) 20-50 wt.% of at least one diacyl glycerol; b) 20-54 wt. % of at least onephosphatidyl choline (PC); c) 5-15 wt. % of at least one biocompatible,organic mono-alcoholic solvent; d) 1-20 wt. % polar solvent; e) 5-150mg/ml of at least one peptide somatostatin receptor agonist comprisingpasireotide; and f) optionally at least one antioxidant; wherein theratio of components a:b is in the range 40:60 to 54:46; wherein thepre-formulation forms, or is capable of forming, at least one liquidcrystalline phase structure upon contact with excess aqueous fluid. 2.The pre-formulation of claim 1, wherein the peptide somatostatinreceptor agonist comprises pasireotide or a salt thereof.
 3. Thepre-formulation of claim 1, wherein the peptide somatostatin receptoragonist consists of pasireotide and optionally octreotide.
 4. Thepre-formulation of claim 1, wherein: the pre-formulation comprises apeptide somatostatin receptor agonist dosage in the range of 10 to 100mg/ml; component a) comprises glycerol dioleate (GDO); component b)comprises soy PC or PC with at least 95% PC head groups and at least 95%C16 to C20 acyl chains having 0 to 3 unsaturations; component c)comprises ethanol, propanol, isopropanol, or mixtures thereof; andcomponent d) comprises water, propylene glycol, or mixtures thereof. 5.(canceled)
 6. (canceled)
 7. (canceled)
 8. (canceled)
 9. Thepre-formulation of claim 1, wherein the antioxidant is ascorbic acid,ascorbyl palmitate, EDTA, or citric acid.
 10. The pre-formulation ofclaim 1, wherein the antioxidant is excluded.
 11. The pre-formulation ofclaim 10, wherein component b) comprises DOPC; DPPC, DSPC; MPPC; MSPC;PMPC; POPC; PSPC; SM PC; SOPC; SPPC; or mixtures thereof.
 12. Thepre-formulation of claim 1, wherein the pre-formulation excludesfragmentation agents.
 13. The pre-formulation of claim 1, wherein:component a) is present at a level of 30-40 wt. %, component b) ispresent at a level of 30-45 wt. %; component c) is present at a level of5-12 wt. %; component d) is present at a level of 1.2-20 wt. %;components c) and d) combined are present at a total level less than orequal to 30 wt. %; the ratio of components a:b is in the range 45:55 to54:46.
 14. (canceled)
 15. (canceled)
 16. (canceled)
 17. (canceled) 18.(canceled)
 19. The pre-formulation of claim 1, wherein d) is water oraqueous fluid and the ratio of components c:d is in the range 40:60 to70:30.
 20. The pre-formulation of claim 1, wherein d) is PG and theratio of components c:d is in the range 90:10 to 25:75.
 21. Thepre-formulation of claim 1, wherein GLP-1, GLP-1 analogues, and GLP-1receptor agonists and/or antagonists are not present.
 22. A pre-filledadministration device containing the pre-formulation of claim
 1. 23. Akit comprising the pre-filled administration device of claim
 22. 24. Aprocess for the formation of a pre-formulation suitable for theadministration of a peptide somatostatin receptor agonist to a subject,the process comprising forming a low viscosity mixture of: a) 20-50 wt.% of at least one diacyl glycerol; b) 20-54 wt. % of at least onephosphatidyl choline (PC); c) 5-15 wt. % of at least one biocompatible,organic mono-alcoholic solvent; d) 1-20 wt. % polar solvent; e) 5-150mg/ml of at least one peptide somatostatin receptor agonist comprisingpasireotide; and f) optionally at least one antioxidant; wherein theratio of components a:b is in the range 40:60 to 54:46; and dissolvingor dispersing at least one peptide somatostatin receptor agonist in thelow viscosity mixture, or in at least one of components a), b), c), d),and optionally f) prior to forming the low viscosity mixture.
 25. Theprocess of claim 24, wherein the peptide somatostatin receptor agonistcomprises pasireotide or a salt thereof.
 26. A method for the treatmentof a human or non-human mammalian subject comprising administering tothe subject the pre-formulation of claim
 1. 27. The method of claim 26for the treatment of a human or non-human mammalian subject in needthereof, wherein the subject has Cushing's disease, acromegaly, type Ior type II diabetes mellitus, and/or complications thereof, irritablebowel syndrome, inflammatory diseases, inflammatory bowel disease,psoriasis or rheumatoid arthritis, polycystic kidney disease, dumpingsyndrome, watery diarrhea syndrome, AIDS-related diarrhea,chemotherapy-induced diarrhea, acute or chronic pancreatitis andgastrointestinal hormone secreting tumors, lymphocyte malignancies, orgastrointestinal bleeding.
 28. The method of claim 26 for the treatmentof a human or non-human mammalian subject in need thereof, wherein thesubject has Cushing's disease or acromegaly.
 29. The method of claim 26comprising administration by a pre-filled administration device.
 30. Themethod of claim 26 comprising a single administration every 20 to 100days.